ArticlePloS one
Expression of 6-Cys Gene Superfamily Defines Babesia bovis Sexual Stage Development within Rhipicephalus microplus.
Article in PloS one. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 35 citations in OpenAlex.
- Structural and antigenic characterization of Babesia Bovis HAP2 domains.Scientific reports · 2025Article
- Current vaccines, experimental immunization trials, and new perspectives to control selected vector borne blood parasites of veterinary importance.Frontiers in veterinary science · 2024Review
- Vaccination of cattle with the Babesia bovis sexual-stage protein HAP2 abrogates parasite transmission by Rhipicephalus microplus ticks.NPJ vaccines · 2023Article
- Differential paired stage-specific expression of Babesia bovis cysteine-rich GCC2/GCC3 domain family proteins (BboGDP) during development within Rhipicephalus microplus.Parasites & vectors · 2023Article
- A TransfectedPathogens (Basel, Switzerland) · 2022Article
- Unraveling the Complexity of the Rhomboid Serine Protease 4 Family ofPathogens (Basel, Switzerland) · 2022Article
- A Culture-Adapted Strain ofFrontiers in cellular and infection microbiology · 2022Article
- Comparison of high throughput RNA sequences betweenFrontiers in cellular and infection microbiology · 2022Article
- Identification of novel immune correlates of protection against acute bovine babesiosis by superinfecting cattle withFrontiers in immunology · 2022Article
- Identification of CCp5 and FNPA as Novel Non-canonical Members of the CCp Protein Family inFrontiers in veterinary science · 2022Article
- Babesiosis as a potential threat for bovine production in China.Parasites & vectors · 2021Review
- Differential expression of calcium-dependent protein kinase 4, tubulin tyrosine ligase, and methyltransferase by xanthurenic acid-induced Babesia bovis sexual stages.Parasites & vectors · 2021Article
- A Comparative Genomic Study of Attenuated and Virulent Strains ofPathogens (Basel, Switzerland) · 2021Article
- HAP2-Mediated Gamete Fusion: Lessons From the World of Unicellular Eukaryotes.Frontiers in cell and developmental biology · 2021Review
- Bovine Babesiosis in Turkey: Impact, Current Gaps, and Opportunities for Intervention.Pathogens (Basel, Switzerland) · 2020Review
- Investigation into limiting dilution and tick transmissibility phenotypes associated with attenuation of the S24 vaccine strain.Parasites & vectors · 2019Article
- Variable and Variant Protein Multigene Families inPathogens (Basel, Switzerland) · 2019Review
- Identification of proteins expressed by Babesia bigemina kinetes.Parasites & vectors · 2019Article
- Unravelling the cellular and molecular pathogenesis of bovine babesiosis: is the sky the limit?International journal for parasitology · 2019Review
- Identification of a putative methyltransferase gene of Babesia bigemina as a novel molecular biomarker uniquely expressed in parasite tick stages.Parasites & vectors · 2018Article
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Babesia bovis, an intra-erythrocytic tick-borne apicomplexan protozoan, is one of the causative agents of bovine babesiosis. Its life cycle includes sexual reproduction within cattle fever ticks, Rhipicephalus spp. Six B. bovis 6-Cys gene superfamily members were previously identified (A, B, C, D, E, F) where their orthologues in Plasmodium parasite have been shown to encode for proteins required for the development of sexual stages. The current study identified four additional 6-Cys genes (G, H, I, J) in the B. bovis genome. These four genes are described in the context of the complete ten 6-Cys gene superfamily. The proteins expressed by this gene family are predicted to be secreted or surface membrane directed. Genetic analysis comparing the 6-Cys superfamily among five distinct B. bovis strains shows limited sequence variation. Additionally, A, B, E, H, I and J genes were transcribed in B. bovis infected tick midgut while genes A, B and E were also transcribed in the subsequent B. bovis kinete stage. Transcription of gene C was found exclusively in the kinete. In contrast, transcription of genes D, F and G in either B. bovis infected midguts or kinetes was not detected. None of the 6-Cys transcripts were detected in B. bovis blood stages. Subsequent protein analysis of 6-Cys A and B is concordant with their transcript profile. The collective data indicate as in Plasmodium parasite, certain B. bovis 6-Cys family members are uniquely expressed during sexual stages and therefore, they are likely required for parasite reproduction. Within B. bovis specifically, proteins encoded by 6-Cys genes A and B are markers for sexual stages and candidate antigens for developing novel vaccines able to interfere with the development of B. bovis within the tick vector.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.