ReviewFrontiers in microbiology2016
Discovery, Molecular Mechanisms, and Industrial Applications of Cold-Active Enzymes.
Review in Frontiers in microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 118 papers.
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Who cites it
118 citing papers in PubMed.
- Discovery of a cryptic aminoacidic triad involved in the temperature adaptation of GH1 enzymes.The FEBS journal · 2026Article
- Decoding the cold: integrated secretome profiling reveals extracellular adaptation mechanisms in Glaciozyma antarctica.Extremophiles : life under extreme conditions · 2026Article
- Partial purification and characterization of an extracellular cold-active protease from Flavobacterium azizsancarii, a novel Antarctic isolate.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2026Article
- Utilization of potato peels waste for production, purification, and characterization of cold-adapted amylase under solid state fermentation by Penicillium goetzii AUMC 498.Microbial cell factories · 2026Article
- Characterisation of a cold-adapted, thermostable glucokinase from psychrophilic Pseudoalteromonas sp. AS-131 reveals how the enzyme achieves high thermal stability without loss of cold adaptation.The FEBS journal · 2026Article
- Mechanisms of Enhanced Low-Temperature Lignocellulose Degradation by an ARTP-InducedMicroorganisms · 2026Article
- Cold-adapted carboxylesterases from Alcanivoracaceae active with a wide range of synthetic polyesters.Applied microbiology and biotechnology · 2026Article
- Discovery and design of novel glucoamylases suitable for raw starch hydrolysis at moderate temperatures via an integrated bioinformatics-assisted strategy.Biotechnology for biofuels and bioproducts · 2026Article
- Mechanistic insights into a cold-adapted glucokinase with high thermal stability were revealed by site-directed spin labeling ESR.Biophysics and physicobiology · 2026Article
- Cold-adaptive variations in the membrane lipid composition in polar species of the ciliateFrontiers in microbiology · 2026Article
- A remote surface loop modulates core structure and cold activity in phosphopantetheine adenylyltransferase.PloS one · 2026Article
- Network reorganization of a cold-adapted β-glucosidase couples conformational flexibility to catalytic efficiency under low-temperature conditions.Frontiers in microbiology · 2026Article
- Enzymatic valorization of fishery by-products: harnessing cold-adapted marine proteases for sustainable production of bioactive peptides.Archives of microbiology · 2025Review
- Enhancing Cold Adaptation of Bidomain Amylases by High-Throughput Computational Engineering.Angewandte Chemie (International ed. in English) · 2025Article
- Sustainable exploitation of high-protein feather waste for green production of cold-adapted and detergent-stable keratinase by Penicillium oxalicum AUMC 15084.World journal of microbiology & biotechnology · 2025Article
- Further Characterization of Lipase B from Ustilago maydis Expressed in Pichia pastoris: a Member of the Candida antarctica Lipase B-like Superfamily.Applied biochemistry and biotechnology · 2025Article
- Expression and characterization of cold-adapted xylanase Xyl-L in Pichia pastoris for xylooligosaccharide (XOS) preparation.Microbial cell factories · 2025Article
- Loop Dynamics Mediate Thermal Adaptation of Two Xylanases from Marine Bacteria.International journal of molecular sciences · 2025Article
- A Low-Temperature-Active Pectate Lyase from a Marine Bacterium for Orange Juice Clarification.Microorganisms · 2025Article
- Novel Antarctic Endo-Polygalacturonase for Pectin Extraction and Vegetal Tissue Maceration at Mild Temperatures.Applied biochemistry and biotechnology · 2025Article
58 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Cold-active enzymes constitute an attractive resource for biotechnological applications. Their high catalytic activity at temperatures below 25°C makes them excellent biocatalysts that eliminate the need of heating processes hampering the quality, sustainability, and cost-effectiveness of industrial production. Here we provide a review of the isolation and characterization of novel cold-active enzymes from microorganisms inhabiting different environments, including a revision of the latest techniques that have been used for accomplishing these paramount tasks. We address the progress made in the overexpression and purification of cold-adapted enzymes, the evolutionary and molecular basis of their high activity at low temperatures and the experimental and computational techniques used for their identification, along with protein engineering endeavors based on these observations to improve some of the properties of cold-adapted enzymes to better suit specific applications. We finally focus on examples of the evaluation of their potential use as biocatalysts under conditions that reproduce the challenges imposed by the use of solvents and additives in industrial processes and of the successful use of cold-adapted enzymes in biotechnological and industrial applications.
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