ArticleMolecular microbiology2016
ClpAP is an auxiliary protease for DnaA degradation in Caulobacter crescentus.
Article in Molecular microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 36 citations in OpenAlex.
- The ClpA chaperone and the two adaptor proteins modulate the fate of the model substrate tagged with a SsrA-degron of Leptospira.The Biochemical journal · 2025Article
- Direct and indirect pathways linking the Lon protease to motility behaviors in the pathogen Pseudomonas aeruginosa.PLoS pathogens · 2025Article
- Live tracking of replisomes reveals nutrient-dependent regulation of replication elongation rates in Caulobacter crescentus.Current biology : CB · 2025Article
- Three factors ParA, TipN, and DnaA-mediated chromosome replication initiation are contributors of centromere segregation inMolecular biology of the cell · 2024Article
- Synchronized Swarmers and Sticky Stalks: Caulobacter crescentus as a Model for Bacterial Cell Biology.Journal of bacteriology · 2023Review
- ATP hydrolysis tunes specificity of a AAA+ protease.Cell reports · 2022Article
- Computational modeling of unphosphorylated CtrA:iScience · 2021Article
- Cryo-EM structure of the full-length Lon protease from Thermus thermophilus.FEBS letters · 2021Article
- Article
- A nascent polypeptide sequence modulates DnaA translation elongation in response to nutrient availability.eLife · 2021Article
- Environmental Conditions Modulate the Transcriptomic Response of BothMicroorganisms · 2021Article
- How Rhizobia Adapt to the Nodule Environment.Journal of bacteriology · 2021Article
- Energy Starvation Induces a Cell Cycle Arrest inFrontiers in molecular biosciences · 2021Article
- The Protein Quality Control Network inFrontiers in molecular biosciences · 2021Review
- Polyphosphate induces the proteolysis of ADP-bound fraction of initiator to inhibit DNA replication initiation upon stress in Escherichia coli.Nucleic acids research · 2020Article
- DNA and Polyphosphate in Directed Proteolysis for DNA Replication Control.Frontiers in microbiology · 2020Review
- Multilayered control of chromosome replication inBiochemical Society transactions · 2019Review
- Lon recognition of the replication initiator DnaA requires a bipartite degron.Molecular microbiology · 2019Article
- Reduction in adaptor amounts establishes degradation hierarchy among protease substrates.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Article
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
The Clp family of proteases is responsible for controlling both stress responses and normal growth. In Caulobacter crescentus, the ClpXP protease is essential and drives cell cycle progression through adaptor-mediated degradation. By contrast, the physiological role for the ClpAP protease is less well understood with only minor growth defects previously reported for ΔclpA cells. Here, we show that ClpAP plays an important role in controlling chromosome content and cell fitness during extended growth. Cells lacking ClpA accumulate aberrant numbers of chromosomes upon prolonged growth suggesting a defect in replication control. Levels of the replication initiator DnaA are elevated in ΔclpA cells and degradation of DnaA is more rapid in cells lacking the ClpA inhibitor ClpS. Consistent with this observation, ClpAP degrades DnaA in vitro while ClpS inhibits this degradation. In cells lacking Lon, the protease previously shown to degrade DnaA in Caulobacter, ClpA overexpression rescues defects in fitness and restores degradation of DnaA. Finally, we show that cells lacking ClpA are particularly sensitive to inappropriate increases in DnaA activity. Our work demonstrates an unexpected effect of ClpAP in directly regulating replication through degradation of DnaA and expands the functional role of ClpAP in Caulobacter.
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