ArticleScientific reports2016
Topographic contrast of ultrathin cryo-sections for correlative super-resolution light and electron microscopy.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 30 citations in OpenAlex.
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- TEM, SEM, and STEM-based immuno-CLEM workflows offer complementary advantages.Scientific reports · 2021Article
- Correlative single-molecule localization microscopy and electron tomography reveals endosome nanoscale domains.Traffic (Copenhagen, Denmark) · 2019Article
- Direct imaging of uncoated biological samples enables correlation of super-resolution and electron microscopy data.Scientific reports · 2018Article
- Automated detection of fluorescent cells in in-resin fluorescence sections for integrated light and electron microscopy.Journal of microscopy · 2018Article
- Correlative imaging of biological tissues with apertureless scanning near-field optical microscopy and confocal laser scanning microscopy.Biomedical optics express · 2017Article
- Article
- Correlative Super-resolution and Electron Microscopy to Resolve Protein Localization in Zebrafish Retina.Journal of visualized experiments : JoVE · 2017Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fluorescence microscopy reveals molecular expression at nanometer resolution but lacks ultrastructural context information. This deficit often hinders a clear interpretation of results. Electron microscopy provides this contextual subcellular detail, but protein identification can often be problematic. Correlative light and electron microscopy produces complimentary information that expands our knowledge of protein expression in cells and tissue. Inherent methodological difficulties are however encountered when combining these two very different microscopy technologies. We present a quick, simple and reproducible method for protein localization by conventional and super-resolution light microscopy combined with platinum shadowing and scanning electron microscopy to obtain topographic contrast from the surface of ultrathin cryo-sections. We demonstrate protein distribution at nuclear pores and at mitochondrial and plasma membranes in the extended topographical landscape of tissue.
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Registered trials
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