ArticleScientific reports2016
Protein characterization of intracellular target-sorted, formalin-fixed cell subpopulations.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Generating Cell Type-Specific Protein Signatures from Non-symptomatic and Diseased Tissues.Annals of biomedical engineering · 2020Pooled it
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- Eplerenone, a mineralocorticoid receptor inhibitor, reduces cirrhosis associated changes of hepatocyte glucose and lipid metabolism.Cell communication and signaling : CCS · 2024Article
- Discovery of surface biomarkers for cell mechanophenotype via an intracellular protein-based enrichment strategy.Cellular and molecular life sciences : CMLS · 2022Article
- Mass-Added Density Modulation for Sorting Cells Based on Differential Surface Protein Levels.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2021Article
- Mesenchymal Stromal Cells Rapidly Suppress TCR Signaling-Mediated Cytokine Transcription in Activated T Cells Through the ICAM-1/CD43 Interaction.Frontiers in immunology · 2021Article
- The dual role of c-src in cell-to-cell transmission of α-synuclein.EMBO reports · 2020Article
- Application of mild hypothermia successfully mitigates neural injury in a 3D in-vitro model of traumatic brain injury.PloS one · 2020Article
- Virus-induced autophagic degradation of STAT2 as a mechanism for interferon signaling blockade.EMBO reports · 2019Article
- Ebola Virus VP40 Modulates Cell Cycle and Biogenesis of Extracellular Vesicles.The Journal of infectious diseases · 2018Article
- Processing fixed and stored adipose-derived stem cells for quantitative protein array assays.BioTechniques · 2017Article
- Influence of Inherent Mechanophenotype on Competitive Cellular Adherence.Annals of biomedical engineering · 2017Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Cellular heterogeneity is inherent in most human tissues, making the investigation of specific cell types challenging. Here, we describe a novel, fixation/intracellular target-based sorting and protein extraction method to provide accurate protein characterization for cell subpopulations. Validation and feasibility tests were conducted using homogeneous, neural cell lines and heterogeneous, rat brain cells, respectively. Intracellular proteins of interest were labeled with fluorescent antibodies for fluorescence-activated cell sorting. Reproducible protein extraction from fresh and fixed samples required lysis buffer with high concentrations of Tris-HCl and sodium dodecyl sulfate as well as exposure to high heat. No deterioration in protein amount or quality was observed for fixed, sorted samples. For the feasibility experiment, a primary rat subpopulation of neuronal cells was selected for based on high, intracellular β-III tubulin signal. These cells showed distinct protein expression differences from the unsorted population for specific (phosphorylated tau) and non-specific (total tau) protein targets. Our approach allows for determining more accurate protein profiles directly from cell types of interest and provides a platform technology in which any cell subpopulation can be biochemically investigated.
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Registered trials
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