ArticleBasic research in cardiology2016
A reproducible protocol for neonatal ischemic injury and cardiac regeneration in neonatal mice.
Article in Basic research in cardiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 40 citations in OpenAlex.
- Assessment of Temperature Monitoring Techniques in Neonatal and Juvenile BALB/cJ and C57BL/6J Mice (Mus musculus) as They Age.Journal of the American Association for Laboratory Animal Science : JAALAS · 2026Article
- Single intramuscular injection of self-amplifying RNA ofScience (New York, N.Y.) · 2026Article
- Critical analysis of descriptive microRNA data in the translational research on cardioprotection and cardiac repair: lost in the complexity of bioinformatics.Basic research in cardiology · 2025Review
- Management of ROS and Regulatory Cell Death in Myocardial Ischemia-Reperfusion Injury.Molecular biotechnology · 2025Review
- Cauterization of the root of the left coronary artery as a straightforward, large and reproducible ischemic injury model in neonatal mice.Lab animal · 2024Review
- Right ventricular cardiomyocyte expansion accompanies cardiac regeneration in newborn mice after large left ventricular infarcts.JCI insight · 2024Article
- Influence of acidic metabolic environment on differentiation of stem cell-derived cardiomyocytes.Frontiers in cardiovascular medicine · 2024Review
- Adult T-cells impair neonatal cardiac regeneration.European heart journal · 2022Article
- CTRP12 alleviates cardiomyocyte ischemia‑reperfusion injury via regulation of KLF15.Molecular medicine reports · 2022Article
- Type 2 innate immunity drives distinct neonatal immune profile conducive for heart regeneration.Theranostics · 2022Article
- Cardiac regeneration: Options for repairing the injured heart.Frontiers in cardiovascular medicine · 2022Review
- A complete heart regeneration model with inflammation as a key component.Experimental animals · 2021Article
- A microRNA program regulates the balance between cardiomyocyte hyperplasia and hypertrophy and stimulates cardiac regeneration.Nature communications · 2021Article
- A surgical mouse model of neonatal pressure overload by transverse aortic constriction.Nature protocols · 2021Article
- Navigating the Crossroads of Cell Therapy and Natural Heart Regeneration.Frontiers in cell and developmental biology · 2021Review
- Microenvironment Stiffness Amplifies Post-ischemia Heart Regeneration in Response to Exogenous Extracellular Matrix Proteins in Neonatal Mice.Frontiers in cardiovascular medicine · 2021Article
- miR-96 and miR-183 differentially regulate neonatal and adult postinfarct neovascularization.JCI insight · 2020Article
- Age-Related Pathways in Cardiac Regeneration: A Role for lncRNAs?Frontiers in physiology · 2020Review
- The cytoprotective impact of yes-associated protein 1 after ischemia-reperfusion injury in AC16 human cardiomyocytes.Experimental biology and medicine (Maywood, N.J.) · 2019Article
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Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cardiac regeneration is one of the prime visions in cardiovascular research. The mouse neonatal apical resection and left anterior descending artery (LAD) ligation model introduced novel in vivo mammalian assays to study cardiac regeneration. However, recent reports and editorials discussed and critically questioned the value and technical reproducibility of the mouse neonatal myocardial infarction approach, making it paramount to develop and use a reproducible model system. We established a mouse neonatal myocardial infarction model by visually confirmed ligation of the LAD using microsurgery. TdT-mediated dUTP nick-end labeling (TUNEL) proved reproducible massive myocardial infarctions in a defined region of the apex and anterior wall of neonatal and 7-day-old mice. Whereas hearts ligated on postnatal day 7 displayed chronic injury, cardiac samples ligated immediately after birth always showed complete structural regeneration after long-term follow-up. Cardiac regeneration was observed in all mouse stains (C57BL/6J, ICR, and mixed background C57BL/6JxSv129) tested so far. We present a detailed in vivo protocol to study complex mechanisms of complete cardiac repair following ischemic cardiac damage. Neonatal LAD ligation surgery is feasible, and results in reproducible myocardial infarctions 24 h after ligation, and no structural myocardial defects are detectable following long-term follow-up. We encourage the cardiovascular community to use our protocol and teaching video to answer key scientific questions in the field of cardiac regeneration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.