Evidence map›Paper›PMID 27664270›Full record

ArticleJournal of experimental botany2016

Alternative splicing of basic chitinase gene PR3b in the low-nicotine mutants of Nicotiana tabacum L. cv. Burley 21.

Haoran Ma, Feng Wang, Wenjing Wang, Guoying Yin, Dingyu Zhang, Yongqiang Ding, Michael P Timko, Hongbo Zhang

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of experimental botany, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact, top 96% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 11 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 2 countries.

Haoran MaTobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao 266101, China College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China.
Feng WangCollege of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China.
Wenjing WangTobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao 266101, China.
Guoying YinCollege of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China.
Dingyu ZhangTobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao 266101, China College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China.
Yongqiang DingTobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao 266101, China College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China.
Michael P TimkoDepartment of Biology, University of Virginia, Charlottesville, Virginia 22904, USA.
Hongbo ZhangTobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao 266101, China zhanghongbo@caas.cn.
Southwest University · CNChinese Academy of Agricultural Sciences · CNTobacco Research Institute · CNUniversity of Virginia · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Two unlinked semi-dominant loci, A (NIC1) and B (NIC2), control nicotine and related alkaloid biosynthesis in Burley tobaccos. Mutations in either or both loci (nic1 and nic2) lead to low nicotine phenotypes with altered environmental stress responses. Here we show that the transcripts derived from the pathogenesis-related (PR) protein gene PR3b are alternatively spliced to a greater extent in the nic1 and nic2 mutants of Burley 21 tobacco and the nic1nic2 double mutant. The alternative splicing results in a deletion of 65 nucleotides and introduces a premature stop codon into the coding region of PR3b that leads to a significant reduction of PR3b specific chitinase activity. Assays of PR3b splicing in F

Indexed as

Alternative SplicingChitinasesCyclopentanesEthylenesGenes, PlantMutationNicotianaNicotineOxylipinsPlant Growth RegulatorsSignal TransductionChitinasesCyclopentanesethyleneEthylenesjasmonic acidNicotineOxylipinsPlant Growth RegulatorsAlternative splicingethylenejasmonatelow-nicotine mutantPR3btobacco.

Identifiers

PMID27664270
PMCPMC5066497
OpenAlexW2522344542

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.