Evidence map›Paper›PMID 27662876›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2017

A Novel Method to Quantify RNA-Protein Interactions In Situ Using FMTRIP and Proximity Ligation.

C Zurla, J Jung, E L Blanchard, P J Santangelo

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.1field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

C ZurlaWallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, 313 Ferst Drive, UA Whitaker Blgd, Atlanta, GA, 30332, USA.
J JungWallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, 313 Ferst Drive, UA Whitaker Blgd, Atlanta, GA, 30332, USA.
E L BlanchardWallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, 313 Ferst Drive, UA Whitaker Blgd, Atlanta, GA, 30332, USA.
P J SantangeloWallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, 313 Ferst Drive, UA Whitaker Blgd, Atlanta, GA, 30332, USA. philip.santangelo@bme.gatech.edu.
Georgia Institute of Technology · USThe Wallace H. Coulter Department of Biomedical Engineering · US

Funding

Graduate Training for Rationally Designed, Integrative Biomaterials - GT BioMATT32EB006343 · NIBIB · GEORGIA INSTITUTE OF TECHNOLOGY · PI TEMENOFF, JOHNNA S · 2008 to 2018
$2.2M
Quantifying native RNA dynamics during regulationR21EB009455 · NIBIB · GEORGIA INSTITUTE OF TECHNOLOGY · PI SANTANGELO, PHILIP J · 2010 to 2011
$399k
NIBIB NIH HHS R21 EB009455NIBIB NIH HHS T32 EB006343
6 · The paper itself

Abstract

RNA binding proteins (RBP) and small RNAs regulate the editing, localization, stabilization, translation, and degradation of ribonucleic acids (RNAs) through their interactions with specific cis-acting elements within target RNAs. Here, we describe a novel method to detect protein-mRNA interactions, which combines FLAG-peptide modified, multiply-labeled tetravalent RNA imaging probes (FMTRIPs) with proximity ligation (PLA), and rolling circle amplification (RCA). This assay detects native RNA in a sequence specific and single RNA sensitive manner, and PLA allows for the quantification and localization of protein-mRNA interactions with single-interaction sensitivity.

Indexed as

AnimalsBiophysical PhenomenaChlorocebus aethiopsHeLa CellsHumansMCF-7 CellsMiceOligopeptidesProtein Interaction MappingRAW 264.7 CellsRNARNA-Binding ProteinsVero CellsFLAG peptideOligopeptidesRNARNA-Binding ProteinsFMTRIPSmRNA binding proteinsPLAPosttranscriptional regulationRCA

Identifiers

PMID27662876
PMCPMC5371434
OpenAlexW2522977003

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.