ArticleeLife2016
A novel optical microscope for imaging large embryos and tissue volumes with sub-cellular resolution throughout.
Article in eLife, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
66 citing papers in PubMed, 180 citations in OpenAlex.
- Mesolens imaging in microbiology.Essays in biochemistry · 2026Review
- Convergent methodologies in prosthetic joint infection research: integrating transdisciplinary approaches to understand and prevent biofilm-driven failure of orthopaedic prostheses.Journal of medical microbiology · 2026Review
- High numerical aperture confocal volumetric mesoscope reveals mesoscale subcellular dynamics in vivo.Nature biotechnology · 2026Article
- Ultra-wide-field, deep, adaptive two-photon microscopy for multi-scale neuronal imaging.Light, science & applications · 2026Article
- Intravital mesoscale optical imaging: challenges, techniques, and future perspectives.Biophysics reports · 2026Article
- Fast Tissue Clearing and Volume Imaging Techniques for Anatomy.Microscopy research and technique · 2025Review
- A cortex-wide multimodal microscope for simultaneous CaNature communications · 2025Article
- PerfectlyAverage: A classical open-source software method to determine the optimal averaging parameters in laser scanning fluorescence microscopy.Journal of microscopy · 2025Article
- A simple silicone elastomer colonization model highlights complexities ofJournal of medical microbiology · 2025Article
- Article
- Mesoscopy: Innovations in high-resolution and large-field imaging.Innovation (Cambridge (Mass.)) · 2025Review
- Imaging 3D cell cultures with optical microscopy.Nature methods · 2025Review
- Evaluating the protective effects of Aurodox in a murine model of Shiga toxin-producing Escherichia coli.npj antimicrobials and resistance · 2025Article
- Principles of microscopy for ophthalmologists.Eye (London, England) · 2025Review
- Article
- Review
- Obtaining super-resolved images at the mesoscale through super-resolution radial fluctuations.Journal of biomedical optics · 2024Article
- An Omni-Mesoscope for multiscale high-throughput quantitative phase imaging of cellular dynamics and high-content molecular characterization.Science advances · 2024Article
- Rapid 3D imaging at cellular resolution for digital cytopathology with a multi-camera array scanner (MCAS).Npj imaging · 2024Article
- A practical guide to light-sheet microscopy for nanoscale imaging: Looking beyond the cell.Journal of applied physics · 2024Article
6 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Current optical microscope objectives of low magnification have low numerical aperture and therefore have too little depth resolution and discrimination to perform well in confocal and nonlinear microscopy. This is a serious limitation in important areas, including the phenotypic screening of human genes in transgenic mice by study of embryos undergoing advanced organogenesis. We have built an optical lens system for 3D imaging of objects up to 6 mm wide and 3 mm thick with depth resolution of only a few microns instead of the tens of microns currently attained, allowing sub-cellular detail to be resolved throughout the volume. We present this lens, called the Mesolens, with performance data and images from biological specimens including confocal images of whole fixed and intact fluorescently-stained 12.5-day old mouse embryos.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.