ArticlePloS one
Development and Evaluation of Real Time RT-PCR Assays for Detection and Typing of Bluetongue Virus.
Article in PloS one. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
48 citing papers in PubMed, 75 citations in OpenAlex.
- Bluetongue virus: current perspectives on characterisation and the need to formalise nomenclature in an era of rapidly evolving technology.The Journal of general virology · 2026Review
- Bluetongue Virus in Cervids in Piedmont During the 2024-2025 Outbreak.Veterinary medicine and science · 2026Article
- Sero-Epidemiology and Molecular Detection of Bluetongue Virus in Goats in Bangladesh.Veterinary medicine and science · 2026Article
- Exceptional Bluetongue Epidemic Caused by Co-Circulation of Several Serotypes in Spain in 2024.Microorganisms · 2026Article
- High Seroprevalence of Bluetongue Virus Serotype 3 in Belgian Cattle and Sheep After the 2024 Epidemic.Viruses · 2026Article
- Detection and serotyping of bluetongue viruses in Khyber Pakhtunkhwa Province, Pakistan.Tropical animal health and production · 2026Article
- Novel multiplex Magpix assay platforms for simultaneous detection of different serotypes of BTV circulating in India.Virus genes · 2026Article
- Optimized library preparation, sequencing, and data analysis protocols for the generation of orbivirus consensus sequences.BMC genomics · 2026Article
- Cross-sectional surveillance of bluetongue virus, epizootic hemorrhagic disease virus, and chronic wasting disease in wild cervids in Nebraska.Frontiers in veterinary science · 2026Article
- Safety and Efficacy of Plant-Produced Trivalent Virus-Like Particle Vaccine Candidate Against Bluetongue Disease.Plant biotechnology journal · 2026Article
- Bluetongue Virus Serotype 3 Follow-Up of the 2024 Outbreak in Two Belgian Zoos.Transboundary and emerging diseases · 2026Article
- Bluetongue in China: Current Status of Viruses, Vectors, Detection Methods, and Vaccines.Transboundary and emerging diseases · 2026Review
- No Evidence of Direct Transmission of Emerging Bluetongue Virus Strains Between Israel and Europe Based on Genomic Analyses (2013-2023).Pathogens (Basel, Switzerland) · 2025Article
- Silent circulation of Bluetongue and Schmallenberg viruses in Culicoides biting midges in northwestern Italy: implications for the 2024 BTV outbreak.Journal of medical entomology · 2025Article
- High seroprevalence and age-associated dynamics of bluetongue and epizootic hemorrhagic disease viruses in North American bison (Frontiers in veterinary science · 2025Article
- Article
- Evaluating Temperature Effects on Bluetongue Virus Serotype 10 and 17 Coinfection inInternational journal of molecular sciences · 2024Article
- Assessing Reassortment between Bluetongue Virus Serotypes 10 and 17 at Different Coinfection Ratios inViruses · 2024Article
- Specific T-cell subsets have a role in anti-viral immunity and pathogenesis but not viral dynamics or onwards vector transmission of an important livestock arbovirus.Frontiers in immunology · 2024Article
- Article
Corrections and comments
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Authors and funding
9 authors at 5 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bluetongue virus is the type species of the genus Orbivirus, family Reoviridae. Bluetongue viruses (BTV) are transmitted between their vertebrate hosts primarily by biting midges (Culicoides spp.) in which they also replicate. Consequently BTV distribution is dependent on the activity, geographic distribution, and seasonal abundance of Culicoides spp. The virus can also be transmitted vertically in vertebrate hosts, and some strains/serotypes can be transmitted horizontally in the absence of insect vectors. The BTV genome is composed of ten linear segments of double-stranded (ds) RNA, numbered in order of decreasing size (Seg-1 to Seg-10). Genome segment 2 (Seg-2) encodes outer-capsid protein VP2, the most variable BTV protein and the primary target for neutralising antibodies. Consequently VP2 (and Seg-2) determine the identity of the twenty seven serotypes and two additional putative BTV serotypes that have been recognised so far. Current BTV vaccines are serotype specific and typing of outbreak strains is required in order to deploy appropriate vaccines. We report development and evaluation of multiple 'TaqMan' fluorescence-probe based quantitative real-time type-specific RT-PCR assays targeting Seg-2 of the 27+1 BTV types. The assays were evaluated using orbivirus isolates from the 'Orbivirus Reference Collection' (ORC) held at The Pirbright Institute. The assays are BTV-type specific and can be used for rapid, sensitive and reliable detection / identification (typing) of BTV RNA from samples of infected blood, tissues, homogenised Culicoides, or tissue culture supernatants. None of the assays amplified cDNAs from closely related but heterologous orbiviruses, or from uninfected host animals or cell cultures.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.