ArticleDisease models & mechanisms2016
An in vitro model of murine middle ear epithelium.
Article in Disease models & mechanisms, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Advances and prospects of 3D air-liquid interface cultured middle ear epithelial cells and inner ear organoids in otologic diseases.Frontiers in bioengineering and biotechnology · 2026Review
- Optimizing synthetic cystic fibrosis sputum media for growth of non-typeable Haemophilus influenzae.Access microbiology · 2025Article
- Inhibition of TGF-β signaling enables long-term proliferation of mouse primary epithelial stem/progenitor cells of the tympanic membrane and the middle ear mucosa.Scientific reports · 2023Article
- Air-liquid interface culture promotes maturation and allows environmental exposure of pluripotent stem cell-derived alveolar epithelium.JCI insight · 2022Article
- Development of a physiological model of human middle ear epithelium.Laryngoscope investigative otolaryngology · 2021Article
- The transcriptional landscape of the cultured murine middle ear epithelium in vitro.Biology open · 2021Article
- Dual RNASeq Reveals NTHi-Macrophage Transcriptomic Changes During Intracellular Persistence.Frontiers in cellular and infection microbiology · 2021Article
- Role of Surfactant Protein D in Experimental Otitis Media.Journal of innate immunity · 2021Article
- Panel 2- recent advance in otitis media bioinformatics.International journal of pediatric otorhinolaryngology · 2020Review
- Panel 4: Recent advances in understanding the natural history of the otitis media microbiome and its response to environmental pressures.International journal of pediatric otorhinolaryngology · 2020Review
- Panel 1: Biotechnology, biomedical engineering and new models of otitis media.International journal of pediatric otorhinolaryngology · 2020Review
- Human primary middle ear epithelial cell culture: A novel in vitro model to study otitis media.Laryngoscope investigative otolaryngology · 2019Article
- A2ML1 and otitis media: novel variants, differential expression, and relevant pathways.Human mutation · 2019Article
- Chronic otitis media is initiated by a bulla cavitation defect in the FBXO11 mouse model.Disease models & mechanisms · 2019Article
- Article
- Loss of the homeostatic protein BPIFA1, leads to exacerbation of otitis media severity in the Junbo mouse model.Scientific reports · 2018Article
- Mapping the distribution of stem/progenitor cells across the mouse middle ear during homeostasis and inflammation.Development (Cambridge, England) · 2018Article
- Review
- The Role of BPIFA1 in Upper Airway Microbial Infections and Correlated Diseases.BioMed research international · 2018Review
- Association of innate defense proteins BPIFA1 and BPIFB1 with disease severity in COPD.International journal of chronic obstructive pulmonary disease · 2018Article
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Authors and funding
11 authors.
Funding
Abstract
Otitis media (OM), or middle ear inflammation, is the most common paediatric disease and leads to significant morbidity. Although understanding of underlying disease mechanisms is hampered by complex pathophysiology it is clear that epithelial abnormalities underpin the disease. There is currently a lack of a well-characterised in vitro model of the middle ear (ME) epithelium that replicates the complex cellular composition of the middle ear. Here, we report the development of a novel in vitro model of mouse middle ear epithelial cells (mMECs) at an air-liquid interface (ALI) that recapitulates the characteristics of the native murine ME epithelium. We demonstrate that mMECs undergo differentiation into the varied cell populations seen within the native middle ear. Proteomic analysis confirmed that the cultures secrete a multitude of innate defence proteins from their apical surface. We showed that the mMECs supported the growth of the otopathogen, nontypeable Haemophilus influenzae (NTHi), suggesting that the model can be successfully utilised to study host-pathogen interactions in the middle ear. Overall, our mMEC culture system can help to better understand the cell biology of the middle ear and improve our understanding of the pathophysiology of OM. The model also has the potential to serve as a platform for validation of treatments designed to reverse aspects of epithelial remodelling that underpin OM development.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.