ArticleClinical oral investigations2017
Acidogenicity of dual-species biofilms of bifidobacteria and Streptococcus mutans.
Article in Clinical oral investigations, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 27 citations in OpenAlex.
- Supragingival plaque microbiota and caries risk factors among children with mixed dentition.BMC oral health · 2025Article
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- Taxonomic and phenotypic analysis of bifidobacteria isolated from IBD patients as potential probiotic strains.BMC microbiology · 2024Article
- The Evolving Microbiome of Dental Caries.Microorganisms · 2024Review
- A comprehensive review of the application of probiotics and postbiotics in oral health.Frontiers in cellular and infection microbiology · 2023Review
- Association between Bifidobacterium and Scardovia Wiggsiae and caries-related factors in severe early childhood caries and caries-free Thai children: a quantitative real-time PCR analysis and a questionnaire cross-sectional study.European archives of paediatric dentistry : official journal of the European Academy of Paediatric Dentistry · 2022Article
- Diversity of site-specific microbes of occlusal and proximal lesions in severe- early childhood caries (S-ECC).Journal of oral microbiology · 2022Article
- Cariogenic Biofilm: Pathology-Related Phenotypes and Targeted Therapy.Microorganisms · 2021Review
- Metagenomic Analysis of Dental Plaque on Pit and Fissure Sites With and Without Caries Among Adolescents.Frontiers in cellular and infection microbiology · 2021Article
- Comparative evaluation of the effects of human breast milk and plain and probiotic-containing infant formulas on enamel mineral content in primary teeth: an in vitro study.European archives of paediatric dentistry : official journal of the European Academy of Paediatric Dentistry · 2020Article
- Article
- Gut microbiota contributes to the distinction between two traditional Chinese medicine syndromes of ulcerative colitis.World journal of gastroenterology · 2019Article
- Correlation between relative bacterial activity and lactate dehydrogenase gene expression of co-cultures in vitro.Clinical oral investigations · 2019Article
- Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveThe aim of this study was to evaluate the acidogenicity of dual-species biofilms of bifidobacteria and Streptococcus mutans. MATERIALS AND
methodsThe following strains were tested: Bifidobacterium dentium DSM20436, Parascardovia denticolens DSM10105, and Scardovia inopinata DSM10107. Streptococcus mutans UA159 and Lactobacillus acidophilus ATCC4356 were used as control. Bifidobacteria were studied planktonically as they were not able to form monospecies biofilm, they were grown in biofilms associated with S. mutans. Endogenous polysaccharide reserves of cultures at log phase were depleted. Standardized suspensions of the microorganisms were incubated in growth media supplemented with 10 mM glucose, lactose, raffinose, glucose, or xylitol. S. mutans biofilms were grown on glass cover slips for 24 h to which bifidobacteria were added. After 24 h, the dual-species biofilms were exposed to the same carbon sources, and after 3 h, the pH of spent culture media and concentrations of organic acids were measured. Statistical analyses were carried out using ANOVA and Tukey's test (α = 0.05).
resultsA higher pH drop was observed when S. mutans was associated with P. denticolens or S. inopinata, in either planktonic or biofilm cultures, than with S. mutans alone. Bifidobacteria showed a higher pH drop in the presence of raffinose than S. mutans or L. acidophilus.
conclusionsDual-species biofilms of bifidobacteria and S. mutans produced more acid and greater pH drops than biofilms of S. mutans alone. CLINICAL RELEVANCE: New insights on the complex process of caries pathogenicity contribute to the establishment of preventive and therapeutic measures, in particular in specific cases, such as in early childhood caries.
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