ArticleJournal of industrial microbiology & biotechnology2016
Genetic switches and related tools for controlling gene expression and electrical outputs of Geobacter sulfurreducens.
Article in Journal of industrial microbiology & biotechnology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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Who cites it
14 citing papers in PubMed, 33 citations in OpenAlex.
- Synthetic microbial co-cultures for modular bioelectronic sensing in diverse environments.Nature biotechnology · 2026Article
- Dynamics of genetic circuits in Pseudomonas protegens.Cell systems · 2026Article
- Synthetic microbial co-cultures for modular bioelectronic sensing in diverse environments.bioRxiv : the preprint server for biology · 2025Article
- An electrogenetic toggle switch model.Microbial biotechnology · 2023Article
- Living electronics: A catalogue of engineered living electronic components.Microbial biotechnology · 2023Review
- Article
- Status quo of tet regulation in bacteria.Microbial biotechnology · 2022Review
- Engineering Biological Electron Transfer and Redox Pathways for Nanoparticle Synthesis.Bioelectricity · 2021Article
- Contextual dependencies expand the re-usability of genetic inverters.Nature communications · 2021Article
- Key Enzymes for Anaerobic Lactate Metabolism in Geobacter sulfurreducens.Applied and environmental microbiology · 2021Article
- Review
- On-going applications of Shewanella species in microbial electrochemical system for bioenergy, bioremediation and biosensing.World journal of microbiology & biotechnology · 2018Review
- Construction of aFrontiers in microbiology · 2018Article
- Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Physiological studies and biotechnology applications of Geobacter species have been limited by a lack of genetic tools. Therefore, potential additional molecular strategies for controlling metabolism were explored. When the gene for citrate synthase, or acetyl-CoA transferase, was placed under the control of a LacI/IPTG regulator/inducer system, cells grew on acetate only in the presence of IPTG. The TetR/AT system could also be used to control citrate synthase gene expression and acetate metabolism. A strain that required IPTG for growth on D-lactate was constructed by placing the gene for D-lactate dehydrogenase under the control of the LacI/IPTG system. D-Lactate served as an inducer in a strain in which a D-lactate responsive promoter and transcription repressor were used to control citrate synthase expression. Iron- and potassium-responsive systems were successfully incorporated to regulate citrate synthase expression and growth on acetate. Linking the appropriate degradation tags on the citrate synthase protein made it possible to control acetate metabolism with either the endogenous ClpXP or exogenous Lon protease and tag system. The ability to control current output from Geobacter biofilms and the construction of an AND logic gate for acetate metabolism suggested that the tools developed may be applicable for biosensor and biocomputing applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.