ArticlePLoS pathogens2016
Impact of Microbiota on Resistance to Ocular Pseudomonas aeruginosa-Induced Keratitis.
Article in PLoS pathogens, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
75 citing papers in PubMed, 1 synthesis or guideline pooled it, 144 citations in OpenAlex.
- What is the impact of microbiota on dry eye: a literature review of the gut-eye axis.BMC ophthalmology · 2024Pooled it
- From Health to Disease: A Comprehensive Review of Ocular Surface Microbiota and Detection Methods in Dry Eye.Current microbiology · 2026Review
- Reduction in Ocular Surface Culture Positivity Following Short-Term Treatment with Liposomal Ozonated Oil Eyedrops.Clinics and practice · 2026Article
- Mechanisms and Safety of Therapeutic Strategies in Meibomian Gland Dysfunction: A Narrative Review.Ophthalmic research · 2026Review
- Helicobacter pylori Infection's Dual Impact on the Ocular Surface: Tear Secretion Disruption and Ocular Surface Microbiome Alteration.Investigative ophthalmology & visual science · 2025Article
- Association Between Ocular Microbiomes of Children and Their Siblings and Parents.Investigative ophthalmology & visual science · 2025Observational
- Multi-cohort analysis identifying core ocular surface microbiome and bacterial alterations in eye diseases.Eye (London, England) · 2025Article
- Alterations of ocular surface microbiome in glaucoma and its association with dry eye.Journal of medical microbiology · 2025Article
- Characterization of Vitreous Microbiota Dysbiosis Associated with Proliferative Diabetic Retinopathy.Diabetes, metabolic syndrome and obesity : targets and therapy · 2025Article
- Unravelling the Microbiome's Role in Healing Diabetic Wounds.Current pharmaceutical biotechnology · 2025Review
- Preliminary exploration of metagenomic sequencing for pathogenic identification in infectious uveitis.Journal of ophthalmic inflammation and infection · 2024Article
- The Ocular Microbiome: Micro-Steps Towards Macro-Shift in Targeted Treatment? A Comprehensive Review.Microorganisms · 2024Review
- Ocular microbiota types and longitudinal microbiota alterations in patients with chronic dacryocystitis with and without antibiotic pretreatment.iMetaOmics · 2024Article
- Changes in the ocular surface microbiome of patients with coronavirus disease 2019 (COVID-19).Frontiers in microbiology · 2024Article
- A Comprehensive Review of Microbial Biofilms on Contact Lenses: Challenges and Solutions.Infection and drug resistance · 2024Review
- Impact of Exposomes on Ocular Surface Diseases.International journal of molecular sciences · 2023Review
- A comparative study on two methods of ocular surface microbial sampling.BMC ophthalmology · 2023Article
- Prenylcysteine oxidase 1 like protein is required for neutrophil bactericidal activities.Nature communications · 2023Article
- Genetic Manipulation of Corynebacterium mastitidis to Better Understand the Ocular Microbiome.Investigative ophthalmology & visual science · 2023Article
- The NLRP3 Inflammasome Is Required for Protection Against Pseudomonas Keratitis.Investigative ophthalmology & visual science · 2023Article
15 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 5 institutions in 2 countries.
Funding
Abstract
The existence of the ocular microbiota has been reported but functional analyses to evaluate its significance in regulating ocular immunity are currently lacking. We compared the relative contribution of eye and gut commensals in regulating the ocular susceptibility to Pseudomonas aeruginosa-induced keratitis. We find that in health, the presence of microbiota strengthened the ocular innate immune barrier by significantly increasing the concentrations of immune effectors in the tear film, including secretory IgA and complement proteins. Consistent with this view, Swiss Webster (SW) mice that are typically resistant to P. aeruginosa-induced keratitis become susceptible due to the lack of microbiota. This was exemplified by increased corneal bacterial burden and elevated pathology of the germ free (GF) mice when compared to the conventionally maintained SW mice. The protective immunity was found to be dependent on both eye and gut microbiota with the eye microbiota having a moderate, but significant impact on the resistance to infection. These events were IL-1ß-dependent as corneal IL-1ß levels were decreased in the infected GF and antibiotic-treated mice when compared to the SPF controls, and neutralization of IL-1ß increased the ocular bacterial burden in the SPF mice. Monocolonizing GF mice with Coagulase Negative Staphylococcus sp. isolated from the conjunctival swabs was sufficient to restore resistance to infection. Cumulatively, these data underline a previously unappreciated role for microbiota in regulating susceptibility to ocular keratitis. We predict that these results will have significant implications for contact lens wearers, where alterations in the ocular commensal communities may render the ocular surface vulnerable to infections.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.