ArticlePLoS computational biology2016
Immersed Boundary Models for Quantifying Flow-Induced Mechanical Stimuli on Stem Cells Seeded on 3D Scaffolds in Perfusion Bioreactors.
Article in PLoS computational biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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Who cites it
11 citing papers in PubMed, 34 citations in OpenAlex.
- Review
- Oxidative stress induces cortical stiffening and cytoskeletal remodelling in pre-apoptotic cancer cells.Cell stress · 2025Article
- Review
- Porous Geometry Guided Micro-mechanical Environment Within Scaffolds for Cell Mechanobiology Study in Bone Tissue Engineering.Frontiers in bioengineering and biotechnology · 2021Review
- Fluid flow-induced cell stimulation in bone tissue engineering changes due to interstitial tissue formation in vitro.International journal for numerical methods in biomedical engineering · 2020Article
- Applications of Computer Modeling and Simulation in Cartilage Tissue Engineering.Tissue engineering and regenerative medicine · 2020Review
- A quantitative high-resolution computational mechanics cell model for growing and regenerating tissues.Biomechanics and modeling in mechanobiology · 2020Article
- A multiscale computational fluid dynamics approach to simulate the micro-fluidic environment within a tissue engineering scaffold with highly irregular pore geometry.Biomechanics and modeling in mechanobiology · 2019Article
- Quantitative cell-based model predicts mechanical stress response of growing tumor spheroids over various growth conditions and cell lines.PLoS computational biology · 2019Article
- Mathematical and computational models for bone tissue engineering in bioreactor systems.Journal of tissue engineeringArticle
- A parameterised mathematical model to elucidate osteoblast cell growth in a phosphate-glass microcarrier culture.Journal of tissue engineeringArticle
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Perfusion bioreactors regulate flow conditions in order to provide cells with oxygen, nutrients and flow-associated mechanical stimuli. Locally, these flow conditions can vary depending on the scaffold geometry, cellular confluency and amount of extra cellular matrix deposition. In this study, a novel application of the immersed boundary method was introduced in order to represent a detailed deformable cell attached to a 3D scaffold inside a perfusion bioreactor and exposed to microscopic flow. The immersed boundary model permits the prediction of mechanical effects of the local flow conditions on the cell. Incorporating stiffness values measured with atomic force microscopy and micro-flow boundary conditions obtained from computational fluid dynamics simulations on the entire scaffold, we compared cell deformation, cortical tension, normal and shear pressure between different cell shapes and locations. We observed a large effect of the precise cell location on the local shear stress and we predicted flow-induced cortical tensions in the order of 5 pN/μm, at the lower end of the range reported in literature. The proposed method provides an interesting tool to study perfusion bioreactors processes down to the level of the individual cell's micro-environment, which can further aid in the achievement of robust bioprocess control for regenerative medicine applications.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.