ArticleNature protocols2016
Efficient genome engineering approaches for the short-lived African turquoise killifish.
Article in Nature protocols, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
What it found
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Who cites it
42 citing papers in PubMed, 87 citations in OpenAlex.
- Loss of killifish cGAS attenuates age-related signatures but does not affect organismal life span.EMBO reports · 2026Article
- An antagonistically pleiotropic gene regulates vertebrate growth, maturity, and lifespan.Nature communications · 2026Article
- A dual role for cGAS in shaping cellular and organismal responses to genomic instability.Genes & development · 2026Article
- A comparative brain atlas in the Mexican cavefish identifies widespread changes in cellular composition and gene expression.bioRxiv : the preprint server for biology · 2026Article
- Molecular evolution of animal aging.The EMBO journal · 2026Review
- First attempt at using a synthetic diet in short-lived killifish, a vertebrate model of ageing.Journal of fish biology · 2026Article
- ER Stress Ire1-Xbp1s Pathway Maintains Youthful Epidermal Basal Layer Through the Regulation of Cell Proliferation.Aging cell · 2025Article
- Enhanced C/EBPα Function Extends Healthspan and Lifespan in the African Turquoise Killifish.Aging cell · 2025Article
- A short-lived fish with long-lasting effects: hallmarks of aging inFrontiers in aging · 2025Review
- The dwarf neon rainbowfish Melanotaenia praecox, a small spiny-rayed fish with potential as a new Acanthomorpha model fish: II. Establishment of a microinjection procedure for genetic engineering.Developmental dynamics : an official publication of the American Association of Anatomists · 2024Article
- Identification of protein aggregates in the aging vertebrate brain with prion-like and phase-separation properties.Cell reports · 2024Article
- Article
- Immune aging in annual killifish.Immunity & ageing : I & A · 2024Review
- The African Turquoise Killifish: A Scalable Vertebrate Model for Aging and Other Complex Phenotypes.Cold Spring Harbor protocols · 2024Article
- Article
- Refeeding-associated AMPKNature aging · 2023Article
- A scalable and tunable platform for functional interrogation of peptide hormones in fish.eLife · 2023Article
- A short dasatinib and quercetin treatment is sufficient to reinstate potent adult neuroregenesis in the aged killifish.NPJ Regenerative medicine · 2023Article
- Article
- Can accelerated ageing models inform us on age-related tauopathies?Aging cell · 2023Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A central challenge in experimental aging research is the lack of short-lived vertebrate models for genetic studies. Here we present a comprehensive protocol for efficient genome engineering in the African turquoise killifish (Nothobranchius furzeri), which is the shortest-lived vertebrate in captivity with a median life span of 4-6 months. By taking advantage of the clustered regularly interspaced short palindromic repeats/CRISPR-associated protein-9 nuclease (CRISPR/Cas9) system and the turquoise killifish genome, this platform enables the generation of knockout alleles via nonhomologous end joining (NHEJ) and knock-in alleles via homology-directed repair (HDR). We include guidelines for guide RNA (gRNA) target design, embryo injection and hatching, germ-line transmission and for minimizing off-target effects. We also provide strategies for Tol2-based transgenesis and large-scale husbandry conditions that are critical for success. Because of the fast life cycle of the turquoise killifish, stable lines can be generated as rapidly as 2-3 months, which is much faster than other fish models. This protocol provides powerful genetic tools for studying vertebrate aging and aging-related diseases.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.