ArticleBiosensors & bioelectronics2017
Multiplexed instrument-free meningitis diagnosis on a polymer/paper hybrid microfluidic biochip.
Article in Biosensors & bioelectronics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers, 2 of them syntheses that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
48 citing papers in PubMed, 2 syntheses or guidelines pooled it, 127 citations in OpenAlex.
- A pooled analysis of the LAMP assay for the detection of Neisseria meningitidis.BMC infectious diseases · 2020Pooled it
- A systematic review of the diagnostic accuracy of Loop-mediated-isothermal AMPlification (LAMP) in the diagnosis of invasive meningococcal disease in children.BMC pediatrics · 2019Pooled it
- Molecular diagnostic methods for rapid diagnosis of central nervous system infections.Frontiers in medical technology · 2025Review
- Multiple Cross Displacement Amplification Combined with Real-Time Fluorescence Monitoring for Efficient, Specific, and SensitiveACS omega · 2024Article
- Microfluidic biosensors for biomarker detection in body fluids: a key approach for early cancer diagnosis.Biomarker research · 2024Review
- Article
- An Integrated Paper Microfluidic Device Based on Isothermal Amplification for Simple Sample-to-Answer Detection ofApplied and environmental microbiology · 2023Article
- CRISPR Assays for Disease Diagnosis: Progress to and Barriers Remaining for Clinical Applications.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Review
- CRISPR-Based Diagnostics and Microfluidics for COVID-19 Point-of-Care Testing: A Review of Main Applications.Molecular biotechnology · 2023Review
- Portable in situ temperature-dependent spectroscopy on a low-cost microfluidic platform integrated with a battery-powered thermofoil heater.View (Beijing, China) · 2023Article
- Rapid quantitation of SARS-CoV-2 antibodies in clinical samples with an electrochemical sensor.Biosensors & bioelectronics · 2023Article
- A portable system for economical nucleic acid amplification testing.Frontiers in bioengineering and biotechnology · 2023Article
- Microfluidic platforms integrated with nano-sensors for point-of-care bioanalysis.Trends in analytical chemistry : TRAC · 2022Article
- Article
- Electrochemical Gene Amplification Signal Detection of Disposable Biochips Using Electrodes.Sensors (Basel, Switzerland) · 2022Article
- Microfluidic technology and its application in the point-of-care testing field.Biosensors & bioelectronics: X · 2022Article
- Molecular Detection of Infectious Laryngotracheitis Virus in Chickens with a Microfluidic Chip.Animals : an open access journal from MDPI · 2021Article
- Recent innovations in cost-effective polymer and paper hybrid microfluidic devices.Lab on a chip · 2021Review
- Emerging point-of-care biosensors for rapid diagnosis of COVID-19: current progress, challenges, and future prospects.Analytical and bioanalytical chemistry · 2021Review
- Multiplex detection of blood-borne pathogens on a self-driven microfluidic chip using loop-mediated isothermal amplification.Analytical and bioanalytical chemistry · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Neisseria meningitidis (N. meningitidis), Streptococcus pneumoniae (S. pneumoniae), and Haemophilus influenzae type b (Hib) are three most common pathogens accounting for most bacterial meningitis, a serious global infectious disease with high fatality, especially in developing nations. Because the treatment and antibiotics differ among each type, the identification of the exact bacteria causing the disease is vital. Herein, we report a polymer/paper hybrid microfluidic biochip integrated with loop-mediated isothermal amplification (LAMP) for multiplexed instrument-free diagnosis of these three major types of bacterial meningitis, with high sensitivity and specificity. Results can be visually observed by the naked eye or imaged by a smartphone camera under a portable UV light source. Without using any specialized laboratory instrument, the limits of detection of a few DNA copies per LAMP zone for N. meningitidis, S. pneumoniae and Hib were achieved within 1h. In addition, these three types of microorganisms spiked in artificial cerebrospinal fluid (ACSF) were directly detected simultaneously, avoiding cumbersome sample preparation procedures in conventional methods. Compared with the paper-free non-hybrid microfluidic biochip over a period of three months, the hybrid microfluidic biochip was found to have a much longer shelf life. Hence, this rapid, instrument-free and highly sensitive microfluidic approach has great potential for point-of-care (POC) diagnosis of multiple infectious diseases simultaneously, especially in resource-limited settings.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.