ArticleJournal of the American Association for Laboratory Animal Science : JAALAS2016
Comparison of Submental Blood Collection with the Retroorbital and Submandibular Methods in Mice (Mus musculus).
Article in Journal of the American Association for Laboratory Animal Science : JAALAS, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 33 citations in OpenAlex.
- CREATINE KINASE IS ELEVATED BY THE SUBMANDIBULAR VEIN BLEED TECHNIQUE, OBSCURING THE MEASUREMENT OF MUSCLE DAMAGE IN SEPSIS.Shock (Augusta, Ga.) · 2025Article
- Circulating CD3Cancer medicine · 2025Article
- Dihydrothiazolo ring-fused 2-pyridone antimicrobial compounds treatScience advances · 2024Article
- Dihydrothiazolo ring-fused 2-pyridone antimicrobial compounds treatbioRxiv : the preprint server for biology · 2024Article
- Evaluation of Submental Blood Collection in Mice (Journal of the American Association for Laboratory Animal Science : JAALAS · 2023Article
- Manipulating adrenergic stress receptor signalling to enhance immunosuppression and prolong survival of vascularized composite tissue transplants.Clinical and translational medicine · 2022Article
- Assessing the Presence of Hematopoietic Stem and Progenitor Cells in Mouse Spleen.Bio-protocol · 2022Article
- A Comparison of Blood Collection Techniques in Mice and their Effects on Welfare.Journal of the American Association for Laboratory Animal Science : JAALAS · 2022Article
- Emvolio - A battery operated portable refrigerator preserves biochemical and haematological integrity of biological samples in preclinical studies.F1000Research · 2022Article
- PCR and RT-PCR in the Diagnosis of Laboratory Animal Infections and in Health Monitoring.Journal of the American Association for Laboratory Animal Science : JAALAS · 2020Article
- A novel approach to genetic engineering of T-cell subsets by hematopoietic stem cell infection with a bicistronic lentivirus.Scientific reports · 2020Article
- The Impact of Common Recovery Blood Sampling Methods, in Mice (Mus Musculus), on Well-Being and Sample Quality: A Systematic Review.Animals : an open access journal from MDPI · 2020Review
- A minimally invasive, low-stress method for serial blood collection in aging mice.Pathobiology of aging & age related diseases · 2019Article
- Comparison of Serial Blood Collection by Facial Vein and Retrobulbar Methods in C57BL/6 Mice.Journal of the American Association for Laboratory Animal Science : JAALAS · 2018Article
- Changes in oxidative stress severity and antioxidant potential during muscle atrophy and reloading in mice.Journal of physical therapy science · 2018Article
- Comparison of Submental Blood Collection with the Retroorbital and Submandibular Methods in Mice (Journal of the American Association for Laboratory Animal Science : JAALAS · 2017Article
Corrections and comments
- Commented on by
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nonterminal blood sample collection of sufficient volume and quality for research is complicated in mice due to their small size and anatomy. Large (>100 μL) nonterminal volumes of unhemolyzed or unclotted blood currently are typically collected from the retroorbital sinus or submandibular plexus. We developed a third method-submental blood collection-which is similar in execution to the submandibular method but with minor changes in animal restraint and collection location. Compared with other techniques, submental collection is easier to perform due to the direct visibility of the target vessels, which are located in a sparsely furred region. Compared with the submandibular method, the submental method did not differ regarding weight change and clotting score but significantly decreased hemolysis and increased the overall number of high-quality samples. The submental method was performed with smaller lancets for the majority of the bleeds, yet resulted in fewer repeat collection attempts, fewer insufficient samples, and less extraneous blood loss and was qualitatively less traumatic. Compared with the retroorbital technique, the submental method was similar regarding weight change but decreased hemolysis, clotting, and the number of overall high-quality samples; however the retroorbital method resulted in significantly fewer incidents of insufficient sample collection. Extraneous blood loss was roughly equivalent between the submental and retroorbital methods. We conclude that the submental method is an acceptable venipuncture technique for obtaining large, nonterminal volumes of blood from mice.
Indexed as
Identifiers
27657712PMC5029828W2553986801What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.