ReviewStem cells international2016
Biological Effects of Culture Substrates on Human Pluripotent Stem Cells.
Review in Stem cells international, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 53 citations in OpenAlex.
- Challenges and solutions in transitioning to animal-free standards: a comprehensive analysis of components in human cell-based developmental neurotoxicity assays.Frontiers in toxicology · 2026Review
- Characterization of bovine and ovine basal-out and apical-out ileum organoids.Royal Society open science · 2025Article
- Article
- A Bioinspired Astrocyte-Derived Coating Promotes the In Vitro Proliferation of Human Neural Stem Cells While Maintaining Their Stemness.Biomimetics (Basel, Switzerland) · 2023Article
- Truncated vitronectin with E-cadherin enables the xeno-free derivation of human embryonic stem cells.Scientific reports · 2023Article
- Droplet microarray platforms for high-throughput drug screening.Mikrochimica acta · 2023Review
- Secreted Protein Acidic and Rich in Cysteine (Diseases (Basel, Switzerland) · 2023Article
- Reduced Cell-ECM Interactions in the EpiSC Colony Center Cause Heterogeneous Differentiation.Cells · 2023Article
- Laminin111-based defined culture promoting self-renewing human pluripotent stem cells with properties of the early post-implantation epiblast.Stem cell reports · 2022Article
- Neural progenitor cell-derived extracellular matrix as a new platform for neural differentiation of human induced pluripotent stem cells.Biomaterials and biosystems · 2022Article
- Toxicological Evaluation of Silver Nanoparticles Synthesized with Peel Extract ofMaterials (Basel, Switzerland) · 2022Article
- In Situ Detection of Kidney Organoid Generation From Stem Cells Using a Simple Electrochemical Method.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Development and evaluation of a novel xeno-free culture medium for human-induced pluripotent stem cells.Stem cell research & therapy · 2022Article
- Miniaturized droplet microarray platform enables maintenance of human induced pluripotent stem cell pluripotency.Materials today. Bio · 2021Article
- Differentiation and Application of Human Pluripotent Stem Cells Derived Cardiovascular Cells for Treatment of Heart Diseases: Promises and Challenges.Frontiers in cell and developmental biology · 2021Review
- Transcriptomic Analysis of Naïve Human Embryonic Stem Cells Cultured in Three-Dimensional PEG Scaffolds.Biomolecules · 2020Article
- Characterization of gene expression changes in human neural stem cells and endothelial cells modeling a neurovascular microenvironment.Brain research bulletin · 2020Article
- Emerging Methods for Enhancing Pluripotent Stem Cell Expansion.Frontiers in cell and developmental biology · 2020Review
- Layer-By-Layer: The Case for 3D Bioprinting Neurons to Create Patient-Specific Epilepsy Models.Materials (Basel, Switzerland) · 2019Review
- Unchain My Heart: Integrins at the Basis of iPSC Cardiomyocyte Differentiation.Stem cells international · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In recent years, as human pluripotent stem cells (hPSCs) have been commonly cultured in feeder-free conditions, a number of cell culture substrates have been applied or developed. However, the functional roles of these substrates in maintaining hPSC self-renewal remain unclear. Here in this review, we summarize the types of these substrates and their effect on maintaining hPSC self-renewal. Endogenous extracellular matrix (ECM) protein expression has been shown to be crucial in maintaining hPSC self-renewal. These ECM molecules interact with integrin cell-surface receptors and transmit their cellular signaling. We discuss the possible effect of integrin-mediated signaling pathways on maintaining hPSC self-renewal. Activation of integrin-linked kinase (ILK), which transmits ECM-integrin signaling to AKT (also known as protein kinase B), has been shown to be critical in maintaining hPSC self-renewal. Also, since naïve pluripotency has been widely recognized as an alternative pluripotent state of hPSCs, we discuss the possible effects of culture substrates and integrin signaling on naïve hPSCs based on the studies of mouse embryonic stem cells. Understanding the role of culture substrates in hPSC self-renewal and differentiation enables us to control hPSC behavior precisely and to establish scalable or microfabricated culture technologies for regenerative medicine and drug development.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.