ArticleGenetics2016
Genetic Mapping by Bulk Segregant Analysis in Drosophila: Experimental Design and Simulation-Based Inference.
Article in Genetics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Early life-stage thermal resilience is determined by climate-linked regulatory variation.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Identification of Critical Candidate Genes Controlling Monokaryon Fruiting inJournal of fungi (Basel, Switzerland) · 2025Article
- Recombinant inbred line panels inform the genetic architecture and interactions of adaptive traits in Drosophila melanogaster.G3 (Bethesda, Md.) · 2025Article
- Rapid identification of a major locus qPRL-C06 affecting primary root length in Brassica napus by QTL-seq.Annals of botany · 2023Article
- The genetic basis of adaptation to copper pollution inFrontiers in genetics · 2023Article
- Evaluation of nine statistics to identify QTLs in bulk segregant analysis using next generation sequencing approaches.BMC genomics · 2022Article
- The evolution of larger size in high-altitude Drosophila melanogaster has a variable genetic architecture.G3 (Bethesda, Md.) · 2022Article
- Predicting the genomic resolution of bulk segregant analysis.G3 (Bethesda, Md.) · 2022Article
- dQTG.seq: A comprehensive R tool for detecting all types of QTLs using extreme phenotype individuals in bi-parental segregation populations.Computational and structural biotechnology journal · 2022Article
- Ethanol resistance inEcology and evolution · 2021Article
- A combined BSA-Seq and linkage mapping approach identifies genomic regions associated with Phytophthora root and crown rot resistance in squash.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2021Article
- Genetic background mutations drive neural circuit hyperconnectivity in a fragile X syndrome model.BMC biology · 2020Article
- Fast genetic mapping of complex traits in C. elegans using millions of individuals in bulk.Nature communications · 2019Article
- Assessing the Architecture ofG3 (Bethesda, Md.) · 2019Article
- Genetic variability affects absolute and relative potencies and kinetics of the anesthetics isoflurane and sevoflurane in Drosophila melanogaster.Scientific reports · 2018Article
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1 author.
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Abstract
Identifying the genomic regions that underlie complex phenotypic variation is a key challenge in modern biology. Many approaches to quantitative trait locus mapping in animal and plant species suffer from limited power and genomic resolution. Here, I investigate whether bulk segregant analysis (BSA), which has been successfully applied for yeast, may have utility in the genomic era for trait mapping in Drosophila (and other organisms that can be experimentally bred in similar numbers). I perform simulations to investigate the statistical signal of a quantitative trait locus (QTL) in a wide range of BSA and introgression mapping (IM) experiments. BSA consistently provides more accurate mapping signals than IM (in addition to allowing the mapping of multiple traits from the same experimental population). The performance of BSA and IM is maximized by having multiple independent crosses, more generations of interbreeding, larger numbers of breeding individuals, and greater genotyping effort, but is less affected by the proportion of individuals selected for phenotypic extreme pools. I also introduce a prototype analysis method for simulation-based inference for BSA mapping (SIBSAM). This method identifies significant QTL and estimates their genomic confidence intervals and relative effect sizes. Importantly, it also tests whether overlapping peaks should be considered as two distinct QTL. This approach will facilitate improved trait mapping in Drosophila and other species for which hundreds or thousands of offspring (but not millions) can be studied.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.