ArticleScientific reports2016
Generation of biallelic knock-out sheep via gene-editing and somatic cell nuclear transfer.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 56 citations in OpenAlex.
- Generation of germline-transmitting transgenic sheep by piggyBac-mediated transgenesis using pronuclear and cytoplasmic gene injection approaches.Transgenic research · 2026Article
- Precise dual-gene knockout of MSTN and SOCS2 via cytidine base editing enhances muscling and growth in goats.Functional & integrative genomics · 2026Article
- Clinical outcome of an SCNT-derivedFrontiers in genome editing · 2026Article
- Molecular markers associated with growth, meat, and carcass traits in sheep: a review.Animal biotechnology · 2025Review
- Genetic Variations ofGenes · 2024Article
- Genome editing: An insight into disease resistance, production efficiency, and biomedical applications in livestock.Functional & integrative genomics · 2024Review
- Current status and future of gene engineering in livestock.BMB reports · 2024Review
- Gene editing in livestock: innovations and applications.Animal reproduction · 2024Article
- Perspectives in Genome-Editing Techniques for Livestock.Animals : an open access journal from MDPI · 2023Review
- Efficient and Specific Generation ofGenes · 2023Article
- Production of MSTN knockout porcine cells using adenine base-editing-mediated exon skipping.In vitro cellular & developmental biology. Animal · 2023Article
- Stem Cell-Based Therapeutic Approaches in Genetic Diseases.Advances in experimental medicine and biology · 2023Article
- Application of Gene Editing Technology in Resistance Breeding of Livestock.Life (Basel, Switzerland) · 2022Review
- Esophageal striated muscle hypertrophy and muscle fiber type transformation in MSTN knockout pigs.Transgenic research · 2022Article
- Genome editing of avian species: implications for animal use and welfare.Laboratory animals · 2022Article
- Advanced tools and methods for single-cell surgery.Microsystems & nanoengineering · 2022Review
- Generation of Heritable Prominent Double Muscle Buttock Rabbits via Novel Site Editing of Myostatin Gene Using CRISPR/Cas9 System.Frontiers in veterinary science · 2022Article
- Efficient Generation of P53 Biallelic Mutations in Diannan Miniature Pigs Using RNA-Guided Base Editing.Life (Basel, Switzerland) · 2021Article
- Efficient correction of a deleterious point mutation in primary horse fibroblasts with CRISPR-Cas9.Scientific reports · 2020Article
- Muscle Hyperplasia in Japanese Quail by Single Amino Acid Deletion in MSTN Propeptide.International journal of molecular sciences · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
23 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transgenic sheep can be used to achieve genetic improvements in breeds and as an important large-animal model for biomedical research. In this study, we generated a TALEN plasmid specific for ovine MSTN and transfected it into fetal fibroblast cells of STH sheep. MSTN biallelic-KO somatic cells were selected as nuclear donor cells for SCNT. In total, cloned embryos were transferred into 37 recipient gilts, 28 (75.7%) becoming pregnant and 15 delivering, resulting in 23 lambs, 12 of which were alive. Mutations in the lambs were verified via sequencing and T7EI assay, and the gene mutation site was consistent with that in the donor cells. Off-target analysis was performed, and no off-target mutations were detected. MSTN KO affected the mRNA expression of MSTN relative genes. The growth curve for the resulting sheep suggested that MSTN KO caused a remarkable increase in body weight compared with those of wild-type sheep. Histological analyses revealed that MSTN KO resulted in muscle fiber hypertrophy. These findings demonstrate the successful generation of MSTN biallelic-KO STH sheep via gene editing in somatic cells using TALEN technology and SCNT. These MSTN mutant sheep developed and grew normally, and exhibited increased body weight and muscle growth.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.