ArticleScientific reports2016
UbC-StarTrack, a clonal method to target the entire progeny of individual progenitors.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed.
- Expanded iOn switch toolkit enables flexible clonal labeling and dynamic imaging in model and non-model animals.Communications biology · 2026Article
- Article
- Genetic Tracing of Progenitors from Embryo to Postnatal Brain.Methods in molecular biology (Clifton, N.J.) · 2025Article
- StarTrack: Mapping Cellular Fates with Inheritable Color Codes.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Dissecting reactive astrocyte responses: lineage tracing and morphology-based clustering.Biological research · 2024Article
- Cell Fate of Retinal Progenitor Cells: In Ovo UbC-StarTrack Analysis.International journal of molecular sciences · 2022Article
- FunctionalNeural regeneration research · 2022Article
- Multicolor strategies for investigating clonal expansion and tissue plasticity.Cellular and molecular life sciences : CMLS · 2022Review
- Visualizing Cortical Development and Evolution: A Toolkit Update.Frontiers in neuroscience · 2022Review
- Lineage Relationships Between Subpallial Progenitors and Glial Cells in the Piriform Cortex.Frontiers in neuroscience · 2022Article
- Article
- Astrocytes and neurons share region-specific transcriptional signatures that confer regional identity to neuronal reprogramming.Science advances · 2021Article
- Deciphering neural heterogeneity through cell lineage tracing.Cellular and molecular life sciences : CMLS · 2021Review
- Epstein-Barr virus-based plasmid enables inheritable transgene expression in mouse cerebral cortex.PloS one · 2021Article
- From Cell States to Cell Fates: How Cell Proliferation and Neuronal Differentiation Are Coordinated During Embryonic Development.Frontiers in neuroscience · 2021Review
- Cortical Interlaminar Astrocytes Are Generated Prenatally, Mature Postnatally, and Express Unique Markers in Human and Nonhuman Primates.Cerebral cortex (New York, N.Y. : 1991) · 2021Article
- Unraveling the adult cell progeny of early postnatal progenitor cells.Scientific reports · 2020Article
- Direct Readout of Neural Stem Cell Transgenesis with an Integration-Coupled Gene Expression Switch.Neuron · 2020Article
- Secretome Analysis of Mesenchymal Stem Cell Factors Fostering Oligodendroglial Differentiation of Neural Stem Cells In Vivo.International journal of molecular sciences · 2020Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Clonal cell analysis defines the potential of single cells and the diversity they can produce. To achieve this, we have developed a novel adaptation of the genetic tracing strategy, UbC-StarTrack, which attributes a specific and unique color-code to single neural precursors, allowing all their progeny to be tracked. We used integrable fluorescent reporters driven by a ubiquitous promoter in PiggyBac-based vectors to achieve inheritable and stable clonal cell labeling. In addition, coupling this to an inducible Cre-LoxP system avoids the expression of non-integrated reporters. To assess the utility of this system, we first analyzed images of combinatorial expression of fluorescent reporters in transfected cells and their progeny. We also validated the efficiency of the UbC-StarTrack to trace cell lineages through in vivo, in vitro and ex vivo strategies. Finally, progenitors located in the lateral ventricles were targeted at embryonic or postnatal stages to determine the diversity of neurons and glia they produce, and their clonal relationships. In this way we demonstrate that UbC-StarTrack can be used to identify all the progeny of a single cell and that it can be employed in a wide range of contexts.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.