ArticlePLoS neglected tropical diseases2016
An Extended Multilocus Sequence Typing (MLST) Scheme for Rapid Direct Typing of Leptospira from Clinical Samples.
Article in PLoS neglected tropical diseases, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Review
- Molecular detection and genetic characterisation of a large flood-borne outbreak of human leptospirosis in Jakarta, Indonesia: A retrospective analysis of surveillance data.PLoS neglected tropical diseases · 2026Article
- Molecular epidemiology of rodent-borneJournal of medical microbiology · 2026Article
- Host-Driven Genetic Diversity ofTransboundary and emerging diseases · 2026Article
- A Guidance for Diagnosis, Control Measures, and Surveillance of Leptospirosis in Non-Human Primates From a Veterinary Perspective in a One-Health Approach.Journal of medical primatology · 2025Review
- Leptospirosis in Unconventional Mammal Pets.Veterinary sciences · 2025Review
- Can Wastewater Surveillance Enhance Genomic Tracking of Climate-Driven Pathogens?Microorganisms · 2025Review
- Leptospirosis Risk Assessment in Rodent Populations and Environmental Reservoirs in Humanitarian Aid Settings in Thailand.Microorganisms · 2024Article
- A probable cluster of premature birth and stillbirth caused by Leptospira interrogans serogroup Hebdomadis in an integrated swine farm in Nagasaki Prefecture, Japan.The Journal of veterinary medical science · 2024Article
- Molecular Detection of Viral and Bacterial Pathogens in Red Foxes (Animals : an open access journal from MDPI · 2024Article
- First isolation and genotyping of pathogenic Leptospira spp. from Austria.Scientific reports · 2024Article
- A Canine Leptospirosis Clinical Case Due toVeterinary sciences · 2023Article
- Core genome sequencing and genotyping of Leptospira interrogans in clinical samples by target capture sequencing.BMC infectious diseases · 2023Article
- Utility evaluation of two molecular methods forHeliyon · 2023Article
- Article
- Culture-Independent Detection and Identification ofMicrobiology spectrum · 2022Article
- Comparison of the Serion IgM ELISA and Microscopic Agglutination Test for diagnosis of Leptospira spp. infections in sera from different geographical origins and estimation of Leptospira seroprevalence in the Wiwa indigenous population from Colombia.PLoS neglected tropical diseases · 2022Article
- Outbreak ofInternational journal of environmental research and public health · 2022Article
- Article
- Article
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRapid typing of Leptospira is currently impaired by requiring time consuming culture of leptospires. The objective of this study was to develop an assay that provides multilocus sequence typing (MLST) data direct from patient specimens while minimising costs for subsequent sequencing. METHODOLOGY AND
findingsAn existing PCR based MLST scheme was modified by designing nested primers including anchors for facilitated subsequent sequencing. The assay was applied to various specimen types from patients diagnosed with leptospirosis between 2014 and 2015 in the United Kingdom (UK) and the Lao Peoples Democratic Republic (Lao PDR). Of 44 clinical samples (23 serum, 6 whole blood, 3 buffy coat, 12 urine) PCR positive for pathogenic Leptospira spp. at least one allele was amplified in 22 samples (50%) and used for phylogenetic inference. Full allelic profiles were obtained from ten specimens, representing all sample types (23%). No nonspecific amplicons were observed in any of the samples. Of twelve PCR positive urine specimens three gave full allelic profiles (25%) and two a partial profile. Phylogenetic analysis allowed for species assignment. The predominant species detected was L. interrogans (10/14 and 7/8 from UK and Lao PDR, respectively). All other species were detected in samples from only one country (Lao PDR: L. borgpetersenii [1/8]; UK: L. kirschneri [1/14], L. santarosai [1/14], L. weilii [2/14]).
conclusionTyping information of pathogenic Leptospira spp. was obtained directly from a variety of clinical samples using a modified MLST assay. This assay negates the need for time-consuming culture of Leptospira prior to typing and will be of use both in surveillance, as single alleles enable species determination, and outbreaks for the rapid identification of clusters.
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