ArticleScientific reports2016
The nuclear genome of Rhazya stricta and the evolution of alkaloid diversity in a medically relevant clade of Apocynaceae.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 37 citations in OpenAlex.
- Ancient gene clusters govern the initiation of monoterpenoid indole alkaloid biosynthesis and C3 stereochemistry inversion.Nature communications · 2025Article
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- Genome and Tissue-Specific Transcriptome of the Tropical Milkweed (Plant direct · 2025Article
- The Madagascar palm genome provides new insights on the evolution of Apocynaceae specialized metabolism.Heliyon · 2024Article
- The Rauvolfia tetraphylla genome suggests multiple distinct biosynthetic routes for yohimbane monoterpene indole alkaloids.Communications biology · 2023Article
- On the complexity of non-binary tree reconciliation with endosymbiotic gene transfer.Algorithms for molecular biology : AMB · 2023Article
- The Vinca minor genome highlights conserved evolutionary traits in monoterpene indole alkaloid synthesis.G3 (Bethesda, Md.) · 2022Article
- Chromosome-level genome assembly of Ophiorrhiza pumila reveals the evolution of camptothecin biosynthesis.Nature communications · 2021Article
- Genome size and identification of repetitive DNA sequences using low coverage sequencing in Hancornia speciosa Gomes (Apocynaceae: Gentianales).Genetics and molecular biology · 2020Article
- Article
- Resolution effects in reconstructing ancestral genomes.BMC genomics · 2018Article
- Genome Assembly and Annotation of the Medicinal PlantG3 (Bethesda, Md.) · 2018Article
- Identification of Phenolic Compounds from Hancornia speciosa (Apocynaceae) Leaves by UHPLC Orbitrap-HRMS.Molecules (Basel, Switzerland) · 2017Article
- Article
- Analysis of transcriptional response to heat stress in Rhazya stricta.BMC plant biology · 2016Article
- Bioinformatic Analyses of Unique (Orphan) Core Genes of the GenusFrontiers in microbiology · 2016Article
Corrections and comments
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Authors and funding
17 authors at 6 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Alkaloid accumulation in plants is activated in response to stress, is limited in distribution and specific alkaloid repertoires are variable across taxa. Rauvolfioideae (Apocynaceae, Gentianales) represents a major center of structural expansion in the monoterpenoid indole alkaloids (MIAs) yielding thousands of unique molecules including highly valuable chemotherapeutics. The paucity of genome-level data for Apocynaceae precludes a deeper understanding of MIA pathway evolution hindering the elucidation of remaining pathway enzymes and the improvement of MIA availability in planta or in vitro. We sequenced the nuclear genome of Rhazya stricta (Apocynaceae, Rauvolfioideae) and present this high quality assembly in comparison with that of coffee (Rubiaceae, Coffea canephora, Gentianales) and others to investigate the evolution of genome-scale features. The annotated Rhazya genome was used to develop the community resource, RhaCyc, a metabolic pathway database. Gene family trees were constructed to identify homologs of MIA pathway genes and to examine their evolutionary history. We found that, unlike Coffea, the Rhazya lineage has experienced many structural rearrangements. Gene tree analyses suggest recent, lineage-specific expansion and diversification among homologs encoding MIA pathway genes in Gentianales and provide candidate sequences with the potential to close gaps in characterized pathways and support prospecting for new MIA production avenues.
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