Evidence map›Paper›PMID 27653519›Full record

ArticleAngewandte Chemie (International ed. in English)2016

Structural and Biochemical Analysis of Protein-Protein Interactions Between the Acyl-Carrier Protein and Product Template Domain.

Jesus F Barajas, Kara Finzel, Timothy R Valentic, Gaurav Shakya, Nathan Gamarra, Delsy Martinez, Jordan L Meier, Anna L Vagstad, Adam G Newman, Craig A Townsend and 2 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Angewandte Chemie (International ed. in English), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
2.1field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 18 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Article
  6. Interfacial plasticity facilitates high reaction rate ofProceedings of the National Academy of Sciences of the United States of America · 2020
    Article
  7. Article
  8. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 5 institutions in 1 country.

Jesus F BarajasDepartment of Molecular Biology and Biochemistry, Chemistry, and Pharmaceutical Sciences, University of California, Irvine, Irvine, CA, 92697, USA.
Kara FinzelDepartment of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA, 92093, USA.
Timothy R ValenticDepartment of Molecular Biology and Biochemistry, Chemistry, and Pharmaceutical Sciences, University of California, Irvine, Irvine, CA, 92697, USA.
Gaurav ShakyaDepartment of Molecular Biology and Biochemistry, Chemistry, and Pharmaceutical Sciences, University of California, Irvine, Irvine, CA, 92697, USA.
Nathan GamarraDepartment of Molecular Biology and Biochemistry, Chemistry, and Pharmaceutical Sciences, University of California, Irvine, Irvine, CA, 92697, USA.
Delsy MartinezDepartment of Molecular Biology and Biochemistry, Chemistry, and Pharmaceutical Sciences, University of California, Irvine, Irvine, CA, 92697, USA.
Jordan L MeierDepartment of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA, 92093, USA.
Anna L VagstadDepartment of Chemistry, The Johns Hopkins University, 3400 North Charles Street, Baltimore, MD, 21218, USA.
Adam G NewmanDepartment of Chemistry, The Johns Hopkins University, 3400 North Charles Street, Baltimore, MD, 21218, USA.
Craig A TownsendDepartment of Chemistry, The Johns Hopkins University, 3400 North Charles Street, Baltimore, MD, 21218, USA.
Michael D BurkartDepartment of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA, 92093, USA. mburkart@ucsd.edu.
Shiou-Chuan TsaiDepartment of Molecular Biology and Biochemistry, Chemistry, and Pharmaceutical Sciences, University of California, Irvine, Irvine, CA, 92697, USA. sctsai@uci.edu.
University of California, Irvine · USUniversity of California, San Diego · USIrvine University · USUniversity of Baltimore · USJohns Hopkins Medicine · US

Funding

Targeting Cancer Through Modifications and MetabolismZIABC011488 · NCI · DIVISION OF BASIC SCIENCES - NCI · PI MEIER, JORDAN L · 2013 to 2025
$21.3M
Iterative Polyketide Synthase Function, Structure and PathwaysR01ES001670 · NIEHS · JOHNS HOPKINS UNIVERSITY · PI TOWNSEND, CRAIG ARTHUR · 1985 to 2023
$9.2M
Protein-Protein Interactions in Natural Product BiosynthesisR01GM095970 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI BURKART, MICHAEL D. · 2012 to 2025
$5.9M
The Chemistry-Biology Interface Program at Johns Hopkins UniversityT32GM080189 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI ROKITA, STEVEN E · 2008 to 2022
$3.5M
The Molecular Basis of Regio-Specificity in Fungal Polyketide SynthaseR01GM100305 · NIGMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI BURKART, MICHAEL D., TSAI, SHIOU-CHUAN · 2012 to 2015
$1.6M
The Ketoreduction and Cyclization of Aromatic Polyketide BiosynthesisR01GM076330 · NIGMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI TSAI, SHIOU-CHUAN · 2007 to 2011
$1.3M
OASIS to identify and analyze natural product biosynthesisR01GM094924 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI BURKART, MICHAEL D. · 2010 to 2013
$1.2M
Regio-Specificities of Fungal Polyketide SynthaseF31GM100738 · NIGMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI BARAJAS, JESUS FEDERICO · 2013 to 2014
$56k
NIEHS NIH HHS R01 ES001670NIGMS NIH HHS F31 GM100738NIGMS NIH HHS R01 GM076330NIGMS NIH HHS R01 GM094924NIGMS NIH HHS R01 GM095970NIGMS NIH HHS R01 GM100305NIGMS NIH HHS T32 GM080189
6 · The paper itself

Abstract

In fungal non-reducing polyketide synthases (NR-PKS) the acyl-carrier protein (ACP) carries the growing polyketide intermediate through iterative rounds of elongation, cyclization and product release. This process occurs through a controlled, yet enigmatic coordination of the ACP with its partner enzymes. The transient nature of ACP interactions with these catalytic domains imposes a major obstacle for investigation of the influence of protein-protein interactions on polyketide product outcome. To further our understanding about how the ACP interacts with the product template (PT) domain that catalyzes polyketide cyclization, we developed the first mechanism-based crosslinkers for NR-PKSs. Through in vitro assays, in silico docking and bioinformatics, ACP residues involved in ACP-PT recognition were identified. We used this information to improve ACP compatibility with non-cognate PT domains, which resulted in the first gain-of-function ACP with improved interactions with its partner enzymes. This advance will aid in future combinatorial biosynthesis of new polyketides.

Indexed as

Acyl Carrier ProteinMolecular ConformationMolecular Docking SimulationPolyketidesProtein BindingProtein ConformationAcyl Carrier ProteinPolyketidesacyl-carrier proteincrosslinkingpolyketide synthaseproduct template domain

Identifiers

PMID27653519
PMCPMC5161458
OpenAlexW2521136690

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.