ArticleBiophysical journal2016
Topography of Cells Revealed by Variable-Angle Total Internal Reflection Fluorescence Microscopy.
Article in Biophysical journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Integrating Lateral Super-Resolution and Axial Progression Reveals Distinct Clathrin Pit Formation Pathways.Traffic (Copenhagen, Denmark) · 2026Article
- Near-wall dynamics of single cavitation bubbles imaged by total internal reflection fluorescence.Ultrasonics sonochemistry · 2025Article
- Membrane topography and the overestimation of protein clustering in single molecule localisation microscopy - identification and correction.Communications biology · 2024Article
- Comparison of thermal and athermal dynamics of the cell membrane slope fluctuations in the presence and absence of Latrunculin-B.Physical biology · 2023Article
- Imaging nanoscale axial dynamics at the basal plasma membrane.The international journal of biochemistry & cell biology · 2023Review
- Nucleation of cadherin clusters on cell-cell interfaces.Scientific reports · 2022Article
- Isotropic three-dimensional dual-color super-resolution microscopy with metal-induced energy transfer.Science advances · 2022Article
- Understanding immune signaling using advanced imaging techniques.Biochemical Society transactions · 2022Review
- Super-resolution imaging: when biophysics meets nanophotonics.Nanophotonics (Berlin, Germany) · 2022Review
- Advanced quantification for single-cell adhesion by variable-angle TIRF nanoscopy.Biophysical reports · 2021Article
- Optometry for a short-sighted microscope.Biophysical journal · 2021Article
- Graphene- and metal-induced energy transfer for single-molecule imaging and live-cell nanoscopy with (sub)-nanometer axial resolution.Nature protocols · 2021Article
- Review
- Large field-of-view nanometer-sectioning microscopy by using metal-induced energy transfer and biexponential lifetime analysis.Communications biology · 2021Article
- High-performance imaging of cell-substrate contacts using refractive index quantification microscopy.Biomedical optics express · 2020Article
- Supercritical Angle Fluorescence Microscopy and Spectroscopy.Biophysical journal · 2020Review
- Variations in Plasma Membrane Topography Can Explain Heterogenous Diffusion Coefficients Obtained by Fluorescence Correlation Spectroscopy.Frontiers in cell and developmental biology · 2020Article
- Interfacial pH Behavior at a Cell/Gate Insulator Nanogap Induced by Allergic Responses.ACS omega · 2019Article
- Calibrating Evanescent-Wave Penetration Depths for Biological TIRF Microscopy.Biophysical journal · 2019Review
- Biologically Coupled Gate Field-Effect Transistors MeetACS omega · 2019Review
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We propose an improved version of variable-angle total internal reflection fluorescence microscopy (vaTIRFM) adapted to modern TIRF setup. This technique involves the recording of a stack of TIRF images, by gradually increasing the incident angle of the light beam on the sample. A comprehensive theory was developed to extract the membrane/substrate separation distance from fluorescently labeled cell membranes. A straightforward image processing was then established to compute the topography of cells with a nanometric axial resolution, typically 10-20 nm. To highlight the new opportunities offered by vaTIRFM to quantify adhesion process of motile cells, adhesion of MDA-MB-231 cancer cells on glass substrate coated with fibronectin was examined.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.