ArticleScience advances2016
Somatic cell reprogramming-free generation of genetically modified pigs.
Article in Science advances, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed.
- Double Electroporation Combined with Zona Pellucida Removal Improves Biallelic Genome Editing Efficiency in Porcine Embryos.Animals : an open access journal from MDPI · 2026Article
- VDGE: a data repository of variation database for gene-edited animals across multiple species.Nucleic acids research · 2025Article
- Genome editing of porcine zygotes via lipofection of two guide RNAs using a CRISPR/Cas9 system.The Journal of reproduction and development · 2024Article
- Artificial Insemination as a Possible Convenient Tool to Acquire Genome-Edited Mice via In Vivo Fertilization with Engineered Sperm.Biotech (Basel (Switzerland)) · 2024Review
- CRISPR Technology Acts as a Dual-Purpose Tool in Pig Breeding: Enhancing Both Agricultural Productivity and Biomedical Applications.Biomolecules · 2024Review
- Generation of allogenic chimera carrying mutations in PDX1 and TP53 genes via phytohemagglutinin-mediated blastomere aggregation in pigs.In vitro cellular & developmental biology. Animal · 2024Article
- Programmed cell death-1-modified pig developed using electroporation-mediated gene editing for in vitro fertilized zygotes.In vitro cellular & developmental biology. Animal · 2024Article
- Evaluation of culture methods and chemical reagent combinations on CRISPR/Cas9 gene editing systems by lipofection in pig zygotes.In vitro cellular & developmental biology. Animal · 2024Article
- Effects of centrifugation treatment before electroporation on gene editing in pig embryos.In vitro cellular & developmental biology. Animal · 2024Article
- One-stepFrontiers in cell and developmental biology · 2024Article
- Gene editing in livestock: innovations and applications.Animal reproduction · 2024Article
- Perspectives in Genome-Editing Techniques for Livestock.Animals : an open access journal from MDPI · 2023Review
- GHR-mutant pig derived from domestic pig and microminipig hybrid zygotes using CRISPR/Cas9 system.Molecular biology reports · 2023Article
- Pigs with anFrontiers in cell and developmental biology · 2023Article
- Multiple gene editing in porcine embryos using a combination of microinjection, electroporation, and transfection methods.Veterinary world · 2022Article
- Large Fragment InDels Reshape Genome Structure of Porcine Alveolar Macrophage 3D4/21 Cells.Genes · 2022Article
- Article
- Effects of the timing of electroporation duringVeterinary and animal science · 2022Article
- Recent Advances in the Production of Genome-Edited Rats.International journal of molecular sciences · 2022Review
- Effect of Aphidicolin, a Reversible Inhibitor of Eukaryotic Nuclear DNA Replication, on the Production of Genetically Modified Porcine Embryos by CRISPR/Cas9.International journal of molecular sciences · 2022Article
Corrections and comments
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Authors and funding
19 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genetically modified pigs for biomedical applications have been mainly generated using the somatic cell nuclear transfer technique; however, this approach requires complex micromanipulation techniques and sometimes increases the risks of both prenatal and postnatal death by faulty epigenetic reprogramming of a donor somatic cell nucleus. As a result, the production of genetically modified pigs has not been widely applied. We provide a simple method for CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 gene editing in pigs that involves the introduction of Cas9 protein and single-guide RNA into in vitro fertilized zygotes by electroporation. The use of gene editing by electroporation of Cas9 protein (GEEP) resulted in highly efficient targeted gene disruption and was validated by the efficient production of Myostatin mutant pigs. Because GEEP does not require the complex methods associated with micromanipulation for somatic reprogramming, it has the potential for facilitating the genetic modification of pigs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.