Evidence map›Paper›PMID 27652036›Full record

ArticleSpringerPlus2016

In vitro culture of rat hair follicle stem cells on rabbit bladder acellular matrix.

Jia Li, Wenguang Wang, Hengqing An, Feng Wang, Mulati Rexiati, Yujie Wang

Open access · diamondAbstract read
In one paragraph

Article in SpringerPlus, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Jia Li *Department of Urology, The First Affiliated Hospital of Xinjiang Medical University, Address: No. 137, Liyushan Road, Urumqi, Xinjiang Uygur Autonomous Region China.
Wenguang Wang *Department of Urology, The First Affiliated Hospital of Xinjiang Medical University, Address: No. 137, Liyushan Road, Urumqi, Xinjiang Uygur Autonomous Region China.
Hengqing AnDepartment of Urology, The First Affiliated Hospital of Xinjiang Medical University, Address: No. 137, Liyushan Road, Urumqi, Xinjiang Uygur Autonomous Region China.
Feng WangDepartment of Urology, The First Affiliated Hospital of Xinjiang Medical University, Address: No. 137, Liyushan Road, Urumqi, Xinjiang Uygur Autonomous Region China.
Mulati RexiatiDepartment of Urology, The First Affiliated Hospital of Xinjiang Medical University, Address: No. 137, Liyushan Road, Urumqi, Xinjiang Uygur Autonomous Region China.
Yujie WangDepartment of Urology, The First Affiliated Hospital of Xinjiang Medical University, Address: No. 137, Liyushan Road, Urumqi, Xinjiang Uygur Autonomous Region China.
First Affiliated Hospital of Xinjiang Medical University · CNXinjiang Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe aim of this work was to create a xenogeneic cell scaffold complex with rabbit bladder acellular matrix and rat hair follicle stem cells, to study the feasibility of construct tissue engineer bladder through biocompatibility of hair follicle stem cells and heterogeneous bladder acellular matrix. MATERIAL AND

methodsNew Zealand rabbit bladder acellular matrix was prepared. Scanning electron microscope and Masson staining were used to analyse the acellular material. Two-steps precipitation method was used to place the third generation of hair follicle stem cells onto the surface of the bladder acellular matrix. The in vitro cell growth on the scaffold complex was regularly monitored through an inverted microscope. Cell growth curve was established and histological examination and scanning electron microscopic were used to analyse the progresses of the cell growth on the matrix material.

resultsThe prepared bladder acellular matrix was white, translucent and membranous. It possessed a fibrous network and collagen structure without any significant cell residues as displayed by the scanning electron microscope, and Masson staining. After 48 h of culture, observation by inverted microscope showed that the hair follicle stem cells grew well around the bladder acellular matrix. After 1 week of culture, scanning electron microscopy showed that the hair follicle stem cells spread and adhered on the surface of the scaffold.

conclusionsThe in vitro culture of rat hair follicle stem cells and the rabbit bladder acellular matrix possessed a good biocompatibility, which provides a good experiment support for hair follicle stem cells to repair the bladder defects disease.

Indexed as

BiocompatibilityBladder acellular matrixCultureHair follicle stem cells

Identifiers

PMID27652036
PMCPMC5007223
OpenAlexW2517803256

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.