Evidence map›Paper›PMID 27652032›Full record

ArticleSpringerPlus2016

Single punch, double biopsy.

Malte Krönig, Norbert Nanko, Vanessa Drendel, Martin Werner, Wolfgang Schultze-Seemann, Anca L Grosu, A Cordula Jilg

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in SpringerPlus, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 94% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Malte KrönigDepartment of Urology, University of Freiburg Medical Centre, Hugstetter Strasse 55, 79106 Freiburg, Germany.
Norbert NankoDepartment of Radiation Oncology, University of Freiburg Medical Centre, Hugstetter Strasse 55, 79106 Freiburg, Germany.
Vanessa DrendelDepartment of Clinical Pathology, University of Freiburg Medical Centre, Breisacher Strasse 155a, 79106 Freiburg, Germany.
Martin WernerDepartment of Clinical Pathology, University of Freiburg Medical Centre, Breisacher Strasse 155a, 79106 Freiburg, Germany.
Wolfgang Schultze-SeemannDepartment of Urology, University of Freiburg Medical Centre, Hugstetter Strasse 55, 79106 Freiburg, Germany.
Anca L GrosuDepartment of Radiation Oncology, University of Freiburg Medical Centre, Hugstetter Strasse 55, 79106 Freiburg, Germany.
A Cordula JilgDepartment of Urology, University of Freiburg Medical Centre, Hugstetter Strasse 55, 79106 Freiburg, Germany.
University Medical Center Freiburg · DEUniversity of Freiburg · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveIn lethal primary metastatic prostate cancer, biopsy material is often the only accessible cancer tissue. Lack of tissue quantity limited the use of biopsy cores for analyzing higher numbers of molecular markers and standard histopathologic evaluation for clinical diagnosis simultaneously. Recent advances in single cell analytics have paved the way to characterize a tumor in more depth from minute input material such as biopsies. We therefore aimed to develop a biopsy needle, which generates two cores side by side from the same punch: one for standard histopathologic analysis to allow for routine diagnostics and the second one for single cell analytics.

methodsOn the basis of a conventional punch biopsy needle we have milled two parallel longitudinal rifts into the needles shat which are separated by a 100 µm thick metal sheet. Each rift can harbor a single tissue core.

resultsTwo cores from the same punch were generated reproducibly from a radical prostatectomy specimen and showed congruent results in histopathologic analysis. Both cores yielded equally sufficient material for standard H&E staining and histopathological evaluation.

conclusionOur modified biopsy system will allow for simultaneous acquisition of tissue cores for diagnostic and scientific analysis from solid tumors or metastatic sites.

Indexed as

Needle biopsyPrimary tissueProstate cancerSingle cellTissue heterogeneity

Identifiers

PMID27652032
PMCPMC5005218
OpenAlexW2513363110

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.