ArticleProceedings of the National Academy of Sciences of the United States of America2016
Dynamic regulation of auxin oxidase and conjugating enzymes AtDAO1 and GH3 modulates auxin homeostasis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers.
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75 citing papers in PubMed, 160 citations in OpenAlex.
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- Comprehensive characterisation of IAA inactivation pathways reveals the impact of glycosylation on auxin metabolism and plant development in Arabidopsis.Communications biology · 2026Article
- Fine mapping and transcriptomic analysis reveal BnaMMS, a novel locus regulating multi-main-stem phenotype via auxin pathway in rapeseed.BMC plant biology · 2026Article
- Cytokinin and auxin metabolism mediation of elevated [COHorticulture research · 2026Article
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- Phenylacetic acid metabolism in land plants: novel pathways and metabolites.Journal of experimental botany · 2025Article
- The mechanism of PGR-free direct organogenesis in Lycium ruthenicum leaf explants revealed by RNA-Seq and phytohormone metabolome co-analysis.BMC plant biology · 2025Article
- Computational modeling of plant root development: the art and the science.The New phytologist · 2025Review
- The ETHYLENE RESPONSE FACTOR6-GRETCHEN HAGEN3.5 module regulates rooting and heat tolerance in Dimocarpus longan.Plant physiology · 2025Article
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- Advances in Plant Auxin Biology: Synthesis, Metabolism, Signaling, Interaction with Other Hormones, and Roles under Abiotic Stress.Plants (Basel, Switzerland) · 2024Review
- Amino acids biosynthesis in root hair development: a mini-review.Biochemical Society transactions · 2024Review
- Multi-scale mechanisms driving root regeneration: From regeneration competence to tissue repatterning.The Plant journal : for cell and molecular biology · 2024Review
- An auxin research odyssey: 1989-2023.The Plant cell · 2024Review
- AtIAR1 is a Zn transporter that regulates auxin metabolism in Arabidopsis thaliana.Journal of experimental botany · 2024Article
- Genome-wide identification and expression characterization of the GH3 gene family of tea plant (Camellia sinensis).BMC genomics · 2024Article
- Morpho-physiological and transcriptomic responses of field pennycress to waterlogging.Frontiers in plant science · 2024Article
- The Roles of GRETCHEN HAGEN3 (GH3)-Dependent Auxin Conjugation in the Regulation of Plant Development and Stress Adaptation.Plants (Basel, Switzerland) · 2023Review
- Comparative Transcriptome Sequencing and Endogenous Phytohormone Content of Annual Grafted Branches ofInternational journal of molecular sciences · 2023Article
15 more citing papers are in PubMed but not listed here.
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13 authors at 3 institutions in 2 countries.
Funding
Abstract
The hormone auxin is a key regulator of plant growth and development, and great progress has been made understanding auxin transport and signaling. Here, we show that auxin metabolism and homeostasis are also regulated in a complex manner. The principal auxin degradation pathways in Arabidopsis include oxidation by Arabidopsis thaliana gene DIOXYGENASE FOR AUXIN OXIDATION 1/2 (AtDAO1/2) and conjugation by Gretchen Hagen3s (GH3s). Metabolic profiling of dao1-1 root tissues revealed a 50% decrease in the oxidation product 2-oxoindole-3-acetic acid (oxIAA) and increases in the conjugated forms indole-3-acetic acid aspartic acid (IAA-Asp) and indole-3-acetic acid glutamic acid (IAA-Glu) of 438- and 240-fold, respectively, whereas auxin remains close to the WT. By fitting parameter values to a mathematical model of these metabolic pathways, we show that, in addition to reduced oxidation, both auxin biosynthesis and conjugation are increased in dao1-1 Transcripts of AtDAO1 and GH3 genes increase in response to auxin over different timescales and concentration ranges. Including this regulation of AtDAO1 and GH3 in an extended model reveals that auxin oxidation is more important for auxin homoeostasis at lower hormone concentrations, whereas auxin conjugation is most significant at high auxin levels. Finally, embedding our homeostasis model in a multicellular simulation to assess the spatial effect of the dao1-1 mutant shows that auxin increases in outer root tissues in agreement with the dao1-1 mutant root hair phenotype. We conclude that auxin homeostasis is dependent on AtDAO1, acting in concert with GH3, to maintain auxin at optimal levels for plant growth and development.
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