ArticleProceedings of the National Academy of Sciences of the United States of America2016
Single-cell dynamics and variability of MAPK activity in a yeast differentiation pathway.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 60 citations in OpenAlex.
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- Global quantitative understanding of non-equilibrium cell fate decision-making in response to pheromone.iScience · 2023Article
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- Pheromone Guidance of Polarity Site Movement in Yeast.Biomolecules · 2022Article
- Polarization and cell-fate decision facilitated by the adaptor Ste50p in Saccharomyces cerevisiae.PloS one · 2022Article
- Exploratory polarization facilitates mating partner selection inMolecular biology of the cell · 2021Article
- A rationally engineered decoder of transient intracellular signals.Nature communications · 2021Article
- Signal-mediated localization of Candida albicans pheromone response pathway components.G3 (Bethesda, Md.) · 2021Article
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- Growth Inhibitory Signaling of the Raf/MEK/ERK Pathway.International journal of molecular sciences · 2020Review
- Dynamically characterizing individual clinical change by the steady state of disease-associated pathway.BMC bioinformatics · 2019Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
In response to pheromones, yeast cells activate a MAPK pathway to direct processes important for mating, including gene induction, cell-cycle arrest, and polarized cell growth. Although a variety of assays have been able to elucidate signaling activities at multiple steps in the pathway, measurements of MAPK activity during the pheromone response have remained elusive, and our understanding of single-cell signaling behavior is incomplete. Using a yeast-optimized FRET-based mammalian Erk-activity reporter to monitor Fus3 and Kss1 activity in live yeast cells, we demonstrate that overall mating MAPK activity exhibits distinct temporal dynamics, rapid reversibility, and a graded dose dependence around the K
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.