ArticleScientific reports2016
Engineering new balancer chromosomes in C. elegans via CRISPR/Cas9.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Separation-of-Function Alleles ofBiomolecules · 2025Article
- Genome sequencing ofGenome research · 2023Article
- Speciation and adaptation research meets genome editing.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Review
- Transgenic tools for targeted chromosome rearrangements allow construction of balancer chromosomes in non-melanogaster Drosophila species.G3 (Bethesda, Md.) · 2022Article
- phiC31 integrase for recombination-mediated single-copy insertion and genome manipulation in Caenorhabditis elegans.Genetics · 2022Article
- Floxed exon (Flexon): A flexibly positioned stop cassette for recombinase-mediated conditional gene expression.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Deciphering complex genome rearrangements in C. elegans using short-read whole genome sequencing.Scientific reports · 2021Article
- Efficient collection of a large number of mutations by mutagenesis of DNA damage response defective animals.Scientific reports · 2021Article
- In vivo CRISPR screening for phenotypic targets of theGenes & development · 2020Article
- Improvement and use of CRISPR/Cas9 to engineer a sperm-marking strain for the invasive fruit pest Drosophila suzukii.BMC biotechnology · 2019Article
- High production of valencene in Saccharomyces cerevisiae through metabolic engineering.Microbial cell factories · 2019Article
- The joy of balancers.PLoS genetics · 2019Review
- TheGenetics · 2019Review
- GRIBCG: a software for selection of sgRNAs in the design of balancer chromosomes.BMC bioinformatics · 2019Article
- Strategies for Efficient Genome Editing Using CRISPR-Cas9.Genetics · 2019Article
- Targeted Chromosomal Rearrangements via Combinatorial Use of CRISPR/Cas9 and Cre/G3 (Bethesda, Md.) · 2018Article
- A Toolkit of Engineered Recombinational Balancers in C. elegans.Trends in genetics : TIG · 2018Article
- Combined flow cytometry and high-throughput image analysis for the study of essential genes in Caenorhabditis elegans.BMC biology · 2018Article
- Comprehensive functional genomics using Caenorhabditis elegans as a model organism.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2017Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Balancer chromosomes are convenient tools used to maintain lethal mutations in heterozygotes. We established a method for engineering new balancers in C. elegans by using the CRISPR/Cas9 system in a non-homologous end-joining mutant. Our studies will make it easier for researchers to maintain lethal mutations and should provide a path for the development of a system that generates rearrangements at specific sites of interest to model and analyse the mechanisms of action of genes.
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Registered trials
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