ArticleScientific reports2016
Photobiomodulation (blue and green light) encourages osteoblastic-differentiation of human adipose-derived stem cells: role of intracellular calcium and light-gated ion channels.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 100 papers, 5 of them syntheses that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
100 citing papers in PubMed, 5 syntheses or guidelines pooled it.
- A Comprehensive Systematic Review of the Effects of Photobiomodulation Therapy in Different Light Wavelength Ranges (Blue, Green, Red, and Near-Infrared) on Sperm Cell Characteristics in Vitro and in Vivo.Reproductive sciences (Thousand Oaks, Calif.) · 2024Pooled it
- The implication of blue light-emitting diode on mesenchymal stem cells: a systematic review.Lasers in medical science · 2023Pooled it
- Enhancing the Therapeutic Potential of Mesenchymal Stem Cells with Light-Emitting Diode: Implications and Molecular Mechanisms.Oxidative medicine and cellular longevity · 2021Pooled it
- NIR light-assisted phototherapies for bone-related diseases and bone tissue regeneration: A systematic review.Theranostics · 2020Pooled it
- Review of light parameters and photobiomodulation efficacy: dive into complexity.Journal of biomedical optics · 2018Pooled it
- Effect Of Photobiomodulation (Low-Level Laser Therapy) In Patients With Knee Osteoarthritis: A Randomized Controlled Trial.Lasers in medical science · 2025Trial
- The effect of combined red, blue, and near-infrared light-emitting diode (LED) photobiomodulation therapy on speed of wound healing after superficial ablative fractional resurfacing.Lasers in medical science · 2024Trial
- Ce3Light: Design, Construction, and Testing of a Light-Irradiation System for In Vitro Cell Studies.Bioengineering (Basel, Switzerland) · 2026Article
- Article
- Differential Effects of 825 nm and 525 nm Photobiomodulation on Neural Differentiation and Mitochondrial Activity in ADSC-Derived Neurospheres.ACS chemical neuroscience · 2026Article
- Immunomodulation Strategies to Enhance Oral Bone Regeneration.Advances in experimental medicine and biology · 2026Review
- The Effect of Transcranial Near-infrared Laser Therapy on Chronic Ischemic Stroke Patients: A Randomized, Single-blind, Sham-controlled Trial.Journal of the Saudi Heart Association · 2026Article
- Photobiomodulation-Driven Tenogenic Differentiation of MSCs in Hydrogel Culture.International journal of molecular sciences · 2025Article
- Photobiomodulation stimulates mitochondrial function and cell proliferation in meniscus-derived stem cells (MeSCs) via activation of TRPV1 channel.Scientific reports · 2025Article
- Optimizing LED photobiomodulation parameters to prevent cartilage matrix degradation in knee osteoarthritis: in vitro and in vivo study.Journal of orthopaedic surgery and research · 2025Article
- Successful Treatment of Vulvar Lichen Sclerosus Using Microfiltered and Preconditioned Suspension of Autologous Adipose-Derived Stem Cells With Photothermal Biomodulation: A Case Report.Clinical case reports · 2025Article
- Advancements in photobiomodulation for generating functional beta cells from adipose derived stem cells in 3D culture: a comprehensive review.Stem cell research & therapy · 2025Review
- [Low-level laser therapy for the treatment of male infertility and erectile dysfunction].Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences · 2025Review
- Article
- Article
40 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
unlabelledHuman adipose-derived stem cells (hASCs) have the potential to differentiate into several different cell types including osteoblasts. Photobiomodulation (PBM) or low level laser therapy (LLLT) using red or near-infrared wavelengths has been reported to have effects on both proliferation and osteogenic differentiation of stem cells. We examined the effects of delivering four different wavelengths (420 nm, 540 nm, 660 nm, 810 nm) at the same dose (3 J/cm(2)) five times (every two days) on hASCs cultured in osteogenic medium over three weeks. We measured expression of the following transcription factors by RT-PCR: RUNX2, osterix, and the osteoblast protein, osteocalcin. The 420 nm and 540 nm wavelengths were more effective in stimulating osteoblast differentiation compared to 660 nm and 810 nm. Intracellular calcium was higher after 420 nm and 540 nm, and could be inhibited by capsazepine and SKF96365, which also inhibited osteogenic differentiation. We hypothesize that activation of light-gated calcium ion channels by blue and green light could explain our results.
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