ArticleAnalytical chemistry2016
Automated, Multiplexed Electrical Impedance Spectroscopy Platform for Continuous Monitoring of Microtissue Spheroids.
Article in Analytical chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Real-Time Monitoring of the Formation and Culture of Hybrid Cell-Microbiomaterial Spheroids Using Non-Faradaic Electrical Impedance Spectroscopy.ACS biomaterials science & engineering · 2025Article
- Seamless integration of CMOS microsensors into open microfluidic systems.Lab on a chip · 2025Article
- Recent Progress in PDMS-Based Microfluidics Toward Integrated Organ-on-a-Chip Biosensors and Personalized Medicine.Biosensors · 2025Review
- Impedance Imaging of Cells and Tissues: Design and Applications.BME frontiers · 2022Article
- What is the future of electrical impedance spectroscopy in flow cytometry?Biomicrofluidics · 2021Article
- Thermosensitive and Conductive Hybrid Polymer for Real-Time Monitoring of Spheroid Growth and Drug Responses.ACS sensors · 2021Article
- Extending In-Plane Impedance Measurements from 2D to 3D Cultures: Design Considerations.Bioengineering (Basel, Switzerland) · 2021Article
- Real-Time Monitoring of Dissection Events of Single Budding Yeast in a Microfluidic Cell-Culturing Device Integrated With Electrical Impedance Biosensor.Frontiers in bioengineering and biotechnology · 2021Article
- Integrating Biosensors in Organs-on-Chip Devices: A Perspective on Current Strategies to Monitor Microphysiological Systems.Biosensors · 2020Review
- Parallelized Wireless Sensing System for Continuous Monitoring of Microtissue Spheroids.ACS sensors · 2020Article
- Parallelized Impedance-Based Platform for Continuous Dose-Response Characterization of Antischistosomal Drugs.Advanced biosystems · 2020Article
- Label-Free Bioelectrochemical Methods for Evaluation of Anticancer Drug Effects at a Molecular Level.Sensors (Basel, Switzerland) · 2020Review
- Integrated Microphysiological Systems: Transferable Organ Models and Recirculating Flow.Advanced biosystems · 2019Review
- Impedance Spectroscopy as a Tool for Monitoring Performance in 3D Models of Epithelial Tissues.Frontiers in bioengineering and biotechnology · 2019Review
- 3D Cardiac Cell Culture: A Critical Review of Current Technologies and Applications.Frontiers in cardiovascular medicine · 2019Review
- Article
- Impedance Spectroscopy and Electrophysiological Imaging of Cells With a High-Density CMOS Microelectrode Array System.IEEE transactions on biomedical circuits and systems · 2018Article
- Smart Cell Culture Systems: Integration of Sensors and Actuators into Microphysiological Systems.ACS chemical biology · 2018Review
- Integrating impedance-based growth-rate monitoring into a microfluidic cell culture platform for live-cell microscopy.Microsystems & nanoengineering · 2018Article
- Applications of Bioimpedance Measurement Techniques in Tissue Engineering.Journal of electrical bioimpedance · 2018Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Microtissue spheroids in microfluidic devices are increasingly used to establish novel in vitro organ models of the human body. As the spheroids are comparably sizable, it is difficult to monitor larger numbers of them by optical means. Therefore, electrical impedance spectroscopy (EIS) emerges as a viable alternative to probing spheroid properties. Current spheroid EIS systems are, however, not suitable for investigating multiple spheroids in parallel over extended time in an automated fashion. Here we address this issue by presenting an automated, multiplexed EIS (AMEIS) platform for impedance analysis in a microfluidic setting. The system was used to continuously monitor the effect of the anticancer drug fluorouracil (5-FU) on HCT116 cancer spheroids. Simultaneous EIS monitoring of up to 15 spheroids was performed in parallel over 4 days at a temporal resolution of 2 min without any need for pumps. The measurements were continuous in nature, and the setup was kept in a standard incubator under controlled conditions during the measurements. A baseline normalization method to improve robustness and to reduce the influence of slow changes in the medium conductivity on the spheroid EIS readings has been developed and validated by experiments and means of a finite-element model. The same method and platform was then used for online monitoring of cardiac spheroids. The beating frequency of each cardiac spheroid could be read out in a completely automated fashion. The developed system constitutes a promising method for simultaneously evaluating drug impact and/or toxic effects on multiple microtissue spheroids.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.