ArticlePharmaceutics2016
Rapid Quantification and Validation of Lipid Concentrations within Liposomes.
Article in Pharmaceutics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 38 citations in OpenAlex.
- Fast and Straightforward Lipid Quantification in Pharmaceutical Compositions Using NMR.ACS omega · 2025Article
- Lipid loss and compositional change during preparation of simple two-component liposomes.Biophysical reports · 2024Article
- Lipid loss and compositional change during preparation of liposomes by common biophysical methods.bioRxiv : the preprint server for biology · 2024Article
- Article
- Preparation and molecular interaction of organic solvent-free piperine pro-liposome from soy lecithin.Heliyon · 2023Article
- Choice of buffer in mobile phase can substantially alter peak areas in quantification of lipids by HPLC-ELSD.Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · 2022Article
- Preparation of DPPC liposomes using probe-tip sonication: Investigating intrinsic factors affecting temperature phase transitions.Biochemistry and biophysics reports · 2020Article
- Translating the fabrication of protein-loaded poly(lactic-co-glycolic acid) nanoparticles from bench to scale-independent production using microfluidics.Drug delivery and translational research · 2020Article
- Sox17 is required for endothelial regeneration following inflammation-induced vascular injury.Nature communications · 2019Article
- Comparative Analysis of Protein Quantification Methods for the Rapid Determination of Protein Loading in Liposomal Formulations.Pharmaceutics · 2019Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Quantification of the lipid content in liposomal adjuvants for subunit vaccine formulation is of extreme importance, since this concentration impacts both efficacy and stability. In this paper, we outline a high performance liquid chromatography-evaporative light scattering detector (HPLC-ELSD) method that allows for the rapid and simultaneous quantification of lipid concentrations within liposomal systems prepared by three liposomal manufacturing techniques (lipid film hydration, high shear mixing, and microfluidics). The ELSD system was used to quantify four lipids: 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), cholesterol, dimethyldioctadecylammonium (DDA) bromide, and ᴅ-(+)-trehalose 6,6'-dibehenate (TDB). The developed method offers rapidity, high sensitivity, direct linearity, and a good consistency on the responses (R² > 0.993 for the four lipids tested). The corresponding limit of detection (LOD) and limit of quantification (LOQ) were 0.11 and 0.36 mg/mL (DMPC), 0.02 and 0.80 mg/mL (cholesterol), 0.06 and 0.20 mg/mL (DDA), and 0.05 and 0.16 mg/mL (TDB), respectively. HPLC-ELSD was shown to be a rapid and effective method for the quantification of lipids within liposome formulations without the need for lipid extraction processes.
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