ArticleInternational journal of molecular sciences2016
Identification of Reference Genes for RT-qPCR Data Normalization in Cannabis sativa Stem Tissues.
Article in International journal of molecular sciences, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 41 citations in OpenAlex.
- Whole-genome analysis reveals the tandem duplication of Cannabis sativa L. GATA and their stress-responsive expression during seed germination.BMC plant biology · 2026Article
- Selection and Validation of Reference Genes inPlants (Basel, Switzerland) · 2024Article
- Characterizing reference genes for high-fidelity gene expression analysis under different abiotic stresses and elicitor treatments in fenugreek leaves.Plant methods · 2024Article
- Challenges toInternational journal of molecular sciences · 2023Article
- A Study on the Use of the Phyto-Courier Technology in Tobacco Leaves Infected byInternational journal of molecular sciences · 2023Article
- Assessing the adaptive role of cannabidiol (CBD) inFrontiers in plant science · 2023Article
- Analysis of Sequence Variability and Transcriptional Profile ofPlants (Basel, Switzerland) · 2021Article
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- Validation of Reference Genes for Quantitative Real-Time PCR Normalization inFrontiers in genetics · 2021Article
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- Molecular and Biochemical Insights Into Early Responses of Hemp to Cd and Zn Exposure and the Potential Effect of Si on Stress Response.Frontiers in plant science · 2021Article
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- Expression of Putative Defense Responses in Cannabis Primed byFrontiers in plant science · 2020Article
- Selection of reference genes for quantitative real-time PCR analysis of photosynthesis-related genes expression inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2019Article
- Article
- Identification of the laccase-like multicopper oxidase gene family of sweet cherry (Prunus avium L.) and expression analysis in six ancient Tuscan varieties.Scientific reports · 2019Article
- Identification of reference genes for RT-qPCR data normalization in Gammarus fossarum (Crustacea Amphipoda).Scientific reports · 2018Article
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- Article
- Identification of fasciclin-like arabinogalactan proteins in textile hemp (Cannabis sativa L.): in silico analyses and gene expression patterns in different tissues.BMC genomics · 2017Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gene expression profiling via quantitative real-time PCR is a robust technique widely used in the life sciences to compare gene expression patterns in, e.g., different tissues, growth conditions, or after specific treatments. In the field of plant science, real-time PCR is the gold standard to study the dynamics of gene expression and is used to validate the results generated with high throughput techniques, e.g., RNA-Seq. An accurate relative quantification of gene expression relies on the identification of appropriate reference genes, that need to be determined for each experimental set-up used and plant tissue studied. Here, we identify suitable reference genes for expression profiling in stems of textile hemp (Cannabis sativa L.), whose tissues (isolated bast fibres and core) are characterized by remarkable differences in cell wall composition. We additionally validate the reference genes by analysing the expression of putative candidates involved in the non-oxidative phase of the pentose phosphate pathway and in the first step of the shikimate pathway. The goal is to describe the possible regulation pattern of some genes involved in the provision of the precursors needed for lignin biosynthesis in the different hemp stem tissues. The results here shown are useful to design future studies focused on gene expression analyses in hemp.
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