ArticleInternational journal of molecular sciences2016
Analysis of Small RNAs in Streptococcus mutans under Acid Stress-A New Insight for Caries Research.
Article in International journal of molecular sciences, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 24 citations in OpenAlex.
- Overexpression of ASFrontiers in cellular and infection microbiology · 2026Article
- Filtration-enriched metabolites and their association with salivary microbiota: a combined two-cohort analysis.Journal of oral microbiology · 2026Article
- Role ofMicroorganisms · 2025Article
- Cordycepin affects Streptococcus mutans biofilm and interferes with its metabolism.BMC oral health · 2025Article
- [Research Updates: Cariogenic Mechanism ofSichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition · 2022Review
- Arginine-induced metabolomic perturbation inJournal of oral microbiology · 2022Article
- [Regulatory role of small RNANan fang yi ke da xue xue bao = Journal of Southern Medical University · 2021Article
- Small regulatory RNAs of oral streptococci and periodontal bacteria.The Japanese dental science review · 2021Review
- Recent Research Advances in Small Regulatory RNAs in Streptococcus.Current microbiology · 2021Review
- Insight into the Effect of Small RNAIndian journal of microbiology · 2019Article
- Effect of Different Glucose Concentrations on Small RNA Levels and Adherence of Streptococcus mutans.Current microbiology · 2019Article
- High-throughput sequencing identification and characterization of potentially adhesion-related small RNAs in Streptococcus mutans.Journal of medical microbiology · 2018Article
- [Changes in expressions of sRNA SpR19 and its potential target GroEL in Streptococcus mutans strains with different cariogenicity cultured under different pH conditions].Nan fang yi ke da xue xue bao = Journal of Southern Medical University · 2017Article
- Effect of the Biofilm Age and Starvation on Acid Tolerance of Biofilm Formed by Streptococcus mutans Isolated from Caries-Active and Caries-Free Adults.International journal of molecular sciences · 2017Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Streptococcus mutans (S. mutans) is the major clinical pathogen responsible for dental caries. Its acid tolerance has been identified as a significant virulence factor for its survival and cariogenicity in acidic conditions. Small RNAs (sRNAs) are recognized as key regulators of virulence and stress adaptation. Here, we constructed three libraries of sRNAs with small size exposed to acidic conditions for the first time, followed by verification using qRT-PCR. The levels of two sRNAs and target genes predicted to be bioinformatically related to acid tolerance were further evaluated under different acid stress conditions (pH 7.5, 6.5, 5.5, and 4.5) at three time points (0.5, 1, and 2 h). Meanwhile, bacterial growth characteristics and vitality were assessed. We obtained 1879 sRNAs with read counts of at least 100. One hundred and ten sRNAs were perfectly mapped to reported msRNAs in S. mutans. Ten out of 18 sRNAs were validated by qRT-PCR. The survival of bacteria declined as the acid was increased from pH 7.5 to 4.5 at each time point. The bacteria can proliferate under each pH except pH 4.5 with time. The levels of sRNAs gradually decreased from pH 7.5 to 5.5, and slightly increased in pH 4.5; however, the expression levels of target mRNAs were up-regulated in acidic conditions than in pH 7.5. These results indicate that some sRNAs are specially induced at acid stress conditions, involving acid adaptation, and provide a new insight into exploring the complex acid tolerance for S. mutans.
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