ArticleThe Biochemical journal1989
Characterization of a cytoskeletal matrix associated with myelin from rat brain.
Article in The Biochemical journal, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 59 citations in OpenAlex.
- Assessment of parental benzo[a]pyrene exposure-induced cross-generational neurotoxicity and changes in offspring sperm DNA methylome in medaka fish.Environmental epigenetics · 2022Article
- Intrinsic and extrinsic regulators of oligodendrocyte progenitor proliferation and differentiation.Seminars in cell & developmental biology · 2021Article
- Melatonin stimulates dendrite formation and complexity in the hilar zone of the rat hippocampus: participation of the Ca++/Calmodulin complex.International journal of molecular sciences · 2015Article
- Interaction of myelin basic protein with cytoskeletal and signaling proteins in cultured primary oligodendrocytes and N19 oligodendroglial cells.BMC research notes · 2014Article
- Myelin management by the 18.5-kDa and 21.5-kDa classic myelin basic protein isoforms.Journal of neurochemistry · 2013Review
- The multiple roles of myelin protein genes during the development of the oligodendrocyte.ASN neuro · 2010Review
- Process outgrowth in oligodendrocytes is mediated by CNP, a novel microtubule assembly myelin protein.The Journal of cell biology · 2005Article
- Lipid rafts in the maintenance of synapses, dendritic spines, and surface AMPA receptor stability.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2003Article
- 2',3'-Cyclic nucleotide 3'-phosphodiesterase: a membrane-bound, microtubule-associated protein and membrane anchor for tubulin.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- An apical-type trafficking pathway is present in cultured oligodendrocytes but the sphingolipid-enriched myelin membrane is the target of a basolateral-type pathway.Molecular biology of the cell · 1998Article
- Microtubule organization and stability in the oligodendrocyte.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1997Article
- Functional implications for the microtubule-associated protein tau: localization in oligodendrocytes.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- In vivo phosphorylation of 2',3'-cyclic nucleotide 3'-phosphohydrolase (CNP): CNP in brain myelin is phosphorylated by forskolin- and phorbol ester-sensitive protein kinases.Neurochemical research · 1994Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Extraction of rat brain myelin in a buffer containing Triton X-100 yielded a soluble fraction and an insoluble residue that was enriched in cytoskeletal elements. Immunoblot analysis of the detergent-soluble fraction and the insoluble cytoskeletal residue showed that all of the tubulin and more than half of the actin were found within the cytoskeletal fraction. The distribution of myelin-specific proteins was also examined, and revealed that 2',3'-cyclic nucleotide 3'-phosphohydrolase (CNPase) I and most of the myelin basic proteins (MBPs) were equally distributed between both fractions. By contrast, the large MBP (21.5 kDa) and CNPase II (50 kDa) were observed to partition almost entirely with the cytoskeletal fraction. Proteolipid protein was found predominantly in the detergent-soluble fraction, as was DM-20 protein. Analysis of the cytoskeletal fraction by sucrose-density-gradient centrifugation demonstrated that a distinct subset of lipids was tightly bound to the cytoskeletal protein residue. The cytoskeleton-associated lipid was considerably enriched in cerebroside and sphingomyelin by comparison with total myelin lipids. These results indicate that a cytoskeletal matrix is associated with multilamellar myelin, and suggest that this structure may play a fundamental role in myelinogenesis.
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