Investigation the Effects of Lactobacillus acidophilus and Lactobacillus casei on aflR Gene expression in Aspergillus parasiticus by Real Time-PCR.

Farzaneh Nateghi, Fatemeh Noorbakhsh, Ensieh Lotfali, Sassan Rezaie

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Article in Iranian journal of public health, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0 citing papers in PubMed, 1 citations in OpenAlex.

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5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Farzaneh NateghiDivision of Microbiology, Dept. of Biology, Islamic Azad University, Varamin-Pishva Branch, Varamin, Iran.

Fatemeh NoorbakhshDivision of Microbiology, Dept. of Biology, Islamic Azad University, Varamin-Pishva Branch, Varamin, Iran.

Ensieh LotfaliDivision of Molecular Biology, Dept. of Medical Mycology & Parasitology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.

Sassan RezaieDivision of Molecular Biology, Dept. of Medical Mycology & Parasitology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.

Islamic Azad University of Varamin · IRTehran University of Medical Sciences · IR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe effect of probiotic bacteria (Lactobacillus acidophilus and L. casei) as safe organisms was examined on fungal growth and aflatoxin gene regulation in Aspergillus parasiticus.

methodsThe fungus was cultured in presence of two different concentrations of L. acidophilus and L. casei in MRS broth medium. Mycelia dry weight is indicated as criteria to evaluate fungal growth. Besides, investigation of aflR gene expression by Real Time PCR was performed for analysis of gene regulatory effects in aflatoxin biosynthetic pathway.

resultsBoth Lactobacillus strongly inhibited fungal growth in the concentrations of 1. 5×10(2), [Formula: see text] . Expression analysis of aflatoxin genes pathway by real time PCR showed inhibitory effect of L. acidophilus and L. casei on expression of aflR gene. The gene expression revealed to be reduced at the approximate rates of 99. 7% and 98% respectively by L. acidopholus and L. casei in concentrations of [Formula: see text] and more.

conclusionL. acidophilus and L. casei may be used successfully as suitable candidates in controlling of A. parasiticus growth on food and feed as well as reducing of aflatoxin contamination.

Indexed as

AflatoxinaflR geneAspergillus parasiticusLactobacilus acidophilusLactobacilus caseiReal Time-PCR

Identifiers

PMID27648422

PMCPMC5026834

OpenAlexW2470525471

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.