Evidence map›Paper›PMID 27648231›Full record

ArticleEcology and evolution2016

Two in one: cryptic species discovered in biological control agent populations using molecular data and crossbreeding experiments.

Iain D Paterson, Rosie Mangan, Douglas A Downie, Julie A Coetzee, Martin P Hill, Ashley M Burke, Paul O Downey, Thomas J Henry, Stephe G Compton

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Ecology and evolution, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Iain D PatersonDepartment of Zoology and Entomology Rhodes University PO Box 94 Grahamstown 6140 South Africa.
Rosie ManganDepartment of Zoology and Entomology Rhodes University PO Box 94 Grahamstown 6140 South Africa.
Douglas A DownieDepartment of Zoology and Entomology Rhodes University PO Box 94 Grahamstown 6140 South Africa.
Julie A CoetzeeDepartment of Zoology and Entomology Rhodes University PO Box 94 Grahamstown 6140 South Africa.
Martin P HillDepartment of Zoology and Entomology Rhodes University PO Box 94 Grahamstown 6140 South Africa.
Ashley M BurkeDepartment of Zoology and Entomology Rhodes University PO Box 94 Grahamstown 6140 South Africa.
Paul O DowneyDepartment of Zoology and Entomology Rhodes University PO Box 94 Grahamstown 6140 South Africa; Institute for Applied Ecology University of Canberra Canberra Australian Capital Territory 2601 Australia.
Thomas J HenrySystematic Entomology Laboratory ARS, USDA, c/o National Museum of Natural History Smithsonian Institution Washington District of Columbia 20013.
Stephe G ComptonDepartment of Zoology and Entomology Rhodes University PO Box 94 Grahamstown 6140 South Africa.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

There are many examples of cryptic species that have been identified through DNA-barcoding or other genetic techniques. There are, however, very few confirmations of cryptic species being reproductively isolated. This study presents one of the few cases of cryptic species that has been confirmed to be reproductively isolated and therefore true species according to the biological species concept. The cryptic species are of special interest because they were discovered within biological control agent populations. Two geographically isolated populations of Eccritotarsus catarinensis (Carvalho) [Hemiptera: Miridae], a biological control agent for the invasive aquatic macrophyte, water hyacinth, Eichhornia crassipes (Mart.) Solms [Pontederiaceae], in South Africa, were sampled from the native range of the species in South America. Morphological characteristics indicated that both populations were the same species according to the current taxonomy, but subsequent DNA analysis and breeding experiments revealed that the two populations are reproductively isolated. Crossbreeding experiments resulted in very few hybrid offspring when individuals were forced to interbreed with individuals of the other population, and no hybrid offspring were recorded when a choice of mate from either population was offered. The data indicate that the two populations are cryptic species that are reproductively incompatible. Subtle but reliable diagnostic characteristics were then identified to distinguish between the two species which would have been considered intraspecific variation without the data from the genetics and interbreeding experiments. These findings suggest that all consignments of biological control agents from allopatric populations should be screened for cryptic species using genetic techniques and that the importation of multiple consignments of the same species for biological control should be conducted with caution.

Indexed as

Biological species conceptC01DNA‐barcodingEccritotarsus catarinensisEichhornia crassipesInter Simple Sequence Repeatsreproductive incompatibility

Identifiers

PMID27648231
PMCPMC5016637

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.