Evidence map›Paper›PMID 27648078›Full record

ArticleStem cells international2016

Optimization of Corneal Epithelial Progenitor Cell Growth on Bombyx mori Silk Fibroin Membranes.

Thomas A Hogerheyde, Shuko Suzuki, Jennifer Walshe, Laura J Bray, Sally A Stephenson, Damien G Harkin, Neil A Richardson

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cells international, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.1field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 12 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Thomas A HogerheydeSchool of Biomedical Sciences, Faculty of Health and Institute of Health & Biomedical Innovation, Queensland University of Technology, 2 George Street, Brisbane, QLD 4001, Australia; Queensland Eye Institute, 140 Melbourne Street, South Brisbane, QLD 4101, Australia.
Shuko SuzukiQueensland Eye Institute, 140 Melbourne Street, South Brisbane, QLD 4101, Australia.
Jennifer WalsheQueensland Eye Institute, 140 Melbourne Street, South Brisbane, QLD 4101, Australia.
Laura J BraySchool of Biomedical Sciences, Faculty of Health and Institute of Health & Biomedical Innovation, Queensland University of Technology, 2 George Street, Brisbane, QLD 4001, Australia; Queensland Eye Institute, 140 Melbourne Street, South Brisbane, QLD 4101, Australia.
Sally A StephensonSchool of Biomedical Sciences, Faculty of Health and Institute of Health & Biomedical Innovation, Queensland University of Technology, 2 George Street, Brisbane, QLD 4001, Australia; Queensland Eye Institute, 140 Melbourne Street, South Brisbane, QLD 4101, Australia.
Damien G HarkinSchool of Biomedical Sciences, Faculty of Health and Institute of Health & Biomedical Innovation, Queensland University of Technology, 2 George Street, Brisbane, QLD 4001, Australia; Queensland Eye Institute, 140 Melbourne Street, South Brisbane, QLD 4101, Australia.
Neil A RichardsonSchool of Biomedical Sciences, Faculty of Health and Institute of Health & Biomedical Innovation, Queensland University of Technology, 2 George Street, Brisbane, QLD 4001, Australia; Queensland Eye Institute, 140 Melbourne Street, South Brisbane, QLD 4101, Australia.ORCID 0000-0002-0729-1483
Queensland University of Technology · AUQueensland Eye Institute · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Scaffolds prepared from silk fibroin derived from cocoons of the domesticated silkworm moth Bombyx mori have demonstrated potential to support the attachment and growth of human limbal epithelial (HLE) cells in vitro. In this study, we attempted to further optimize protocols to promote the expansion of HLE cells on B. mori silk fibroin- (BMSF-) based scaffolds. BMSF films were initially coated with different extracellular matrix proteins and then analysed for their impact on corneal epithelial cell adhesion, cell morphology, and culture confluency. Results showed that collagen I, collagen III, and collagen IV consistently improved HCE-T cell adherence, promoted an elongated cell morphology, and increased culture confluency. By contrast, ECM coating had no significant effect on the performance of primary HLE cells cultured on BMSF films. In the second part of this study, primary HLE cells were grown on BMSF films in the presence of medium (SHEM) supplemented with keratinocyte growth factor (KGF) and the Rho kinase inhibitor, Y-27632. The results demonstrated that SHEM medium supplemented with KGF and Y-27632 dramatically increased expression of corneal differentiation markers, keratin 3 and keratin 12, whereas expression of the progenitor marker, p63, did not appear to be significantly influenced by the choice of culture medium.

Identifiers

PMID27648078
PMCPMC5018328
OpenAlexW2508190033

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.