Evidence map›Paper›PMID 27648002›Full record

ArticleInternational journal of medical sciences2016

Regulatory SNPs Alter the Gene Expression of Diabetic Retinopathy Associated Secretary Factors.

Chian-Feng Chen, Shiow-Wen Liou, Hsin-Han Wu, Chin-Hui Lin, Li-Shan Huang, Lin-Chung Woung, Ching-Yao Tsai

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of medical sciences, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
2.1field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 18 citations in OpenAlex.

  1. Article
  2. Polymorphisms inBiomedicines · 2025
    Review
  3. Genetic Variability inInternational journal of molecular sciences · 2023
    Article
  4. Article
  5. Association between theOpen life sciences · 2023
    Article
  6. Article
  7. Article
  8. Genetics of Diabetic Retinopathy.Current diabetes reports · 2019
    Review
  9. Oncotarget · 2017
    Article
  10. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 5 institutions in 1 country.

Chian-Feng ChenVYM Genome Research Center, National Yang-Ming University, Taipei, Taiwan;
Shiow-Wen LiouDepartment of Ophthalmology, Taipei City Hospital, Taipei, Taiwan;; Department of Ophthalmology, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan;; Department of Ophthalmology, Taipei Medical University, Taipei, Taiwan;; Department of Ophthalmology, Taiwan University Hospital, Taipei, Taiwan;
Hsin-Han WuVYM Genome Research Center, National Yang-Ming University, Taipei, Taiwan;
Chin-Hui LinVYM Genome Research Center, National Yang-Ming University, Taipei, Taiwan;
Li-Shan HuangDepartment of Internal Medicine, Taipei City Hospital, Taipei, Taiwan;
Lin-Chung WoungDepartment of Ophthalmology, Taipei City Hospital, Taipei, Taiwan;; Department of Ophthalmology, Taiwan University Hospital, Taipei, Taiwan;; Department of Health Care Management, National Taipei University of Nursing and Health Sciences, Taipei, Taiwan;; Department of Medicine, National Yang-Ming University, Taipei, Taiwan;; Institute of Public Health, National Yang-Ming University, Taipei, Taiwan.
Ching-Yao TsaiDepartment of Ophthalmology, Taipei City Hospital, Taipei, Taiwan;; Institute of Public Health, National Yang-Ming University, Taipei, Taiwan.
National Yang Ming Chiao Tung University · TWNational Taiwan University · TWNational Yang Ming University Hospital · TWTaipei City Hospital · TWTaipei Medical University · TW

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectivesDiabetic retinopathy (DR) is a common microvascular complication in both type I and type II diabetes. Several previous reports indicated the serum centration of some secretary factors were highly associated with DR. Therefore, we hypothesis regulatory SNPs (rSNPs) genotype in secretary factors may alter these gene expression and lead to DR.

methodsAt first, pyrosequencing were applying to screen the SNPs which present allele frequency different in DR and DNR. Then individual genotyping was processed by Taqman assays in Taiwanese DR and DNR patients. To evaluate the effect of SNP allele on transcriptional activity, we measured promoter activity using luciferase reporter constructs.

resultsWe found the frequencies of the CC, CG, and GG genotype of the rs2010963 polymorphism were 15.09%, 47.14%, and 37.74% in DR and 12.90%, 19.35%, and 67.74% in DNR, respectively (p = 0.0205). The prevalence of DR was higher (p = 0.00793) in patients with the CC or CG genotype (62.26% and 32.26% for DR and DNR, respectively) compared with the patients with the GG genotype. To evaluate the effect of rs2010963-C allele on transcriptional activity, we measured promoter activity using luciferase reporter constructs. The rs2010963-C reporter showed 1.6 to 2-fold higher luciferase activity than rs2010963-G in 3 cell lines.

conclusionOur data proposed rs2010963-C altered the expression level of VEGFA in different tissues. We suggested small increase but long term exposure to VEGFA may lead to DR finally.

Indexed as

AdultDiabetes Mellitus, Type 2Diabetic RetinopathyFemaleGene Expression RegulationGene FrequencyGenetic Association StudiesGenetic Predisposition to DiseaseGenotypeHumansMaleMiddle AgedPolymorphism, Single NucleotideRegulatory Sequences, Nucleic AcidRisk FactorsVascular Endothelial Growth Factor AVascular Endothelial Growth Factor AVEGFA protein, humanDRSNPVEGFA.

Identifiers

PMID27648002
PMCPMC5027191
OpenAlexW2518844085

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.