ArticleProceedings of the National Academy of Sciences of the United States of America2016
Molecular organization of cytokinesis nodes and contractile rings by super-resolution fluorescence microscopy of live fission yeast.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 84 papers.
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Who cites it
84 citing papers in PubMed.
- The Ync13-Rga7-Rng10 complex selectively coordinates secretory vesicle trafficking and secondary septum formation during cytokinesis.PLoS biology · 2025Article
- CD99-mediated immunological synapse formation potentiates CAR-T cell function.Nature communications · 2025Article
- Spatial control of secretory vesicle targeting by the Ync13-Rga7-Rng10 complex during cytokinesis.bioRxiv : the preprint server for biology · 2025Article
- Mechanisms of force transmission on cytokinesis Formin Cdc12 in fission yeast revealed by coiled-coil force sensors.bioRxiv : the preprint server for biology · 2025Article
- Structural and functional mechanisms of actin isoforms.The FEBS journal · 2025Review
- Regulation of epithelial cell jamming transition by cytoskeleton and cell-cell interactions.Biophysics reviews · 2024Review
- The Myosin-V Myo51 and Alpha-Actinin Ain1p Cooperate during Contractile Ring Assembly and Disassembly in Fission Yeast Cytokinesis.Journal of fungi (Basel, Switzerland) · 2024Article
- Measurement precision bounds on aberrated single-molecule emission patterns.Optics express · 2024Article
- Processes Controlling the Contractile Ring during Cytokinesis in Fission Yeast, Including the Role of ESCRT Proteins.Journal of fungi (Basel, Switzerland) · 2024Review
- Anillin-related Mid1 as an adaptive and multimodal contractile ring anchoring protein: A simulation study.Structure (London, England : 1993) · 2024Article
- Emergence of periodic circumferential actin cables from the anisotropic fusion of actin nanoclusters during tubulogenesis.Nature communications · 2024Article
- Intracellular tension sensor reveals mechanical anisotropy of the actin cytoskeleton.Nature communications · 2023Article
- Anillin forms linear structures and facilitates furrow ingression after septin and formin depletion.Cell reports · 2023Article
- Unraveling the kinetochore nanostructure in Schizosaccharomyces pombe using multi-color SMLM imaging.The Journal of cell biology · 2023Article
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- Actin turnover protects the cytokinetic contractile ring from structural instability.Journal of cell science · 2023Article
- Non-muscle myosin 2 at a glance.Journal of cell science · 2023Review
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- Imp2p forms actin-dependent clusters and imparts stiffness to the contractile ring.Molecular biology of the cell · 2022Article
24 more citing papers are in PubMed but not listed here.
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5 authors.
Funding
Abstract
Cytokinesis in animals, fungi, and amoebas depends on the constriction of a contractile ring built from a common set of conserved proteins. Many fundamental questions remain about how these proteins organize to generate the necessary tension for cytokinesis. Using quantitative high-speed fluorescence photoactivation localization microscopy (FPALM), we probed this question in live fission yeast cells at unprecedented resolution. We show that nodes, protein assembly precursors to the contractile ring, are discrete structural units with stoichiometric ratios and distinct distributions of constituent proteins. Anillin Mid1p, Fes/CIP4 homology-Bin/amphiphysin/Rvs (F-BAR) Cdc15p, IQ motif containing GTPase-activating protein (IQGAP) Rng2p, and formin Cdc12p form the base of the node that anchors the ends of myosin II tails to the plasma membrane, with myosin II heads extending into the cytoplasm. This general node organization persists in the contractile ring where nodes move bidirectionally during constriction. We observed the dynamics of the actin network during cytokinesis, starting with the extension of short actin strands from nodes, which sometimes connected neighboring nodes. Later in cytokinesis, a broad network of thick bundles coalesced into a tight ring around the equator of the cell. The actin ring was ∼125 nm wide and ∼125 nm thick. These observations establish the organization of the proteins in the functional units of a cytokinetic contractile ring.
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