ArticleProceedings of the National Academy of Sciences of the United States of America2016
Dynamics of Escherichia coli's passive response to a sudden decrease in external osmolarity.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
- Review
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- Osmotic stress triggers fast and reversible PMF collapse in Escherichia coli.Biophysical journal · 2026Article
- Hypoosmolarity inhibits ammonia oxidation by terrestrial and freshwater Nitrosopumilaceae members.The ISME journal · 2026Article
- Unlocking Ectoine's Postbiotic Therapeutic Promise: Mechanisms, Applications, and Future Directions.Probiotics and antimicrobial proteins · 2026Review
- Stress-mediated growth determinesProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Article
- Morphogenesis of bacterial cables in polymeric environments.Science advances · 2025Article
- Its own architect: Flipping cardiolipin synthase.Science advances · 2025Article
- Hypo-osmotic stress is an anticipatory trigger of heat-resistance in presumptive extraintestinal pathogenicFrontiers in microbiology · 2025Article
- Ethanolamine-induced assembly of microcompartments is required forbioRxiv : the preprint server for biology · 2024Article
- Quantifying the fractal complexity of nutrient transport channels inMicrobiology (Reading, England) · 2024Article
- Observing mechanosensitive channels in action in living bacteria.Biophysical reports · 2024Article
- Macromolecular Crowding, Phase Separation, and Homeostasis in the Orchestration of Bacterial Cellular Functions.Chemical reviews · 2024Review
- Cell envelope growth of Gram-negative bacteria proceeds independently of cell wall synthesis.The EMBO journal · 2023Article
- Physicochemical homeostasis in bacteria.FEMS microbiology reviews · 2023Article
- Article
- Mechanosensitive channel MscS is critical for termination of the bacterial hypoosmotic permeability response.The Journal of general physiology · 2023Article
- Bladder irrigation with tap water to reduce antibiotic treatment for catheter-associated urinary tract infections: an evaluation of clinical practice.Frontiers in urology · 2023Article
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7 authors.
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Abstract
For most cells, a sudden decrease in external osmolarity results in fast water influx that can burst the cell. To survive, cells rely on the passive response of mechanosensitive channels, which open under increased membrane tension and allow the release of cytoplasmic solutes and water. Although the gating and the molecular structure of mechanosensitive channels found in Escherichia coli have been extensively studied, the overall dynamics of the whole cellular response remain poorly understood. Here, we characterize E. coli's passive response to a sudden hypoosmotic shock (downshock) on a single-cell level. We show that initial fast volume expansion is followed by a slow volume recovery that can end below the initial value. Similar response patterns were observed at downshocks of a wide range of magnitudes. Although wild-type cells adapted to osmotic downshocks and resumed growing, cells of a double-mutant ([Formula: see text]) strain expanded, but failed to fully recover, often lysing or not resuming growth at high osmotic downshocks. We propose a theoretical model to explain our observations by simulating mechanosensitive channels opening, and subsequent solute efflux and water flux. The model illustrates how solute efflux, driven by mechanical pressure and solute chemical potential, competes with water influx to reduce cellular osmotic pressure and allow volume recovery. Our work highlights the vital role of mechanosensation in bacterial survival.
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