Evidence map›Paper›PMID 27645053›Full record

ReviewNucleus (Austin, Tex.)2016

The proto-chromatosome: A fundamental subunit of chromatin?

Josefina Ocampo, Feng Cui, Victor B Zhurkin, David J Clark

Open access · goldAbstract readReview
PubMed Full text at PMC Publisher
In one paragraph

Review in Nucleus (Austin, Tex.), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.3field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Josefina Ocampoa Division of Developmental Biology, Eunice Kennedy Shriver National Institute for Child Health and Human Development, National Institutes of Health , Bethesda , MD , USA.
Feng Cuib Thomas H. Gosnell School of Life Sciences, Rochester Institute of Technology , Rochester , NY , USA.
Victor B Zhurkinc National Cancer Institute, National Institutes of Health , Bethesda , MD , USA.
David J Clarka Division of Developmental Biology, Eunice Kennedy Shriver National Institute for Child Health and Human Development, National Institutes of Health , Bethesda , MD , USA.
National Institutes of Health · USRochester Institute of Technology · US

Funding

Chromatin Remodeling and Gene ActivationZIAHD008775 · NICHD · EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT · PI CLARK, DAVID J · 2009 to 2025
$20.1M
Uncovering a new role of nucleosomes in gene regulationR15GM116102 · NIGMS · ROCHESTER INSTITUTE OF TECHNOLOGY · PI CUI, FENG · 2015 to 2019
$883k
NIGMS NIH HHS R15 GM116102
6 · The paper itself

Abstract

Eukaryotic DNA is packaged into regularly spaced nucleosomes, resembling beads on a string. Each bead contains ∼147 bp wrapped around a core histone octamer. Linker histone (H1) binds to the linker DNA to drive chromatin folding. Micrococcal nuclease (MNase) digestion studies reveal 2 mono-nucleosomal intermediates: the core particle (∼147 bp) and the chromatosome (∼160 bp; a core particle with additional DNA protected by H1). We have recently developed an improved method for mapping nucleosomes, using exonuclease III to remove residual linker (MNase-Exo-seq). (1) We discovered 2 new intermediate particles corresponding to core particles with ∼7 bp of linker protruding from one side (∼154 bp) or both sides (∼161 bp), which are formed in the absence of H1. We propose that these "proto-chromatosomes" are stabilized by core histone-DNA contacts in the linker, ∼7 bp from the nucleosome boundaries. These contacts may determine the topography of the H1 binding site.

Indexed as

ChromatinDNAHistonesMicrococcal NucleaseNucleosomesChromatinDNAHistonesMicrococcal NucleaseNucleosomeschromatin foldingchromatosomelinker histoneMNase-Exo-seqMnase-seqnucleosomenucleosome mappingproto-chromatosome

Identifiers

PMID27645053
PMCPMC5039001
OpenAlexW2516211505

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.