ArticleMolecular & cellular proteomics : MCP2016
Comparative Ploidy Proteomics of Candida albicans Biofilms Unraveled the Role of the AHP1 Gene in the Biofilm Persistence Against Amphotericin B.
Article in Molecular & cellular proteomics : MCP, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Antifungal persistence: Clinical relevance and mechanisms.PLoS pathogens · 2025Review
- Review
- The role of Npt1 in regulating antifungal protein activity in filamentous fungi.Nature communications · 2025Article
- Sdd3 regulates the biofilm formation ofmBio · 2025Article
- Differential carbon source utilization drives metabolic state and resuscitation in antibiotic-tolerant persister cells.Frontiers in pharmacology · 2025Article
- Exploring therapeutic avenues: mesenchymal stem/stromal cells and exosomes in confronting enigmatic biofilm-producing fungi.Archives of microbiology · 2023Review
- Mixed Fungal Biofilms: From Mycobiota to Devices, a New Challenge on Clinical Practice.Microorganisms · 2022Review
- Inactivating the mannose-ethanolamine phosphotransferase Gpi7 confers caspofungin resistance in the human fungal pathogenCell surface (Amsterdam, Netherlands) · 2021Article
- Article
- Review
- Studying Bacterial Persistence: Established Methods and Current Advances.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Transcription Factors of CAT1, EFG1, and BCR1 Are Effective in Persister Cells ofFrontiers in microbiology · 2021Article
- The Proteome of Community LivingMicroorganisms · 2020Article
- Oxidative Imbalance inFrontiers in microbiology · 2020Article
- Article
- Multi-omics Analyses Reveal Synergistic Carbohydrate Metabolism in Streptococcus mutans-Candida albicans Mixed-Species Biofilms.Infection and immunity · 2019Article
- Mass Spectrometry-Based Proteomics of Fungal Pathogenesis, Host-Fungal Interactions, and Antifungal Development.Journal of fungi (Basel, Switzerland) · 2019Review
- Article
- The effects of cigarette smoking and exercise on total salivary antioxidant activity.The Saudi dental journal · 2019Article
- Fungal persister cells: The basis for recalcitrant infections?PLoS pathogens · 2018Review
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Candida albicans is a major fungal pathogen causing lethal infections in immunocompromised patients. C. albicans forms antifungal tolerant biofilms contributing significantly to therapeutic failure. The recently established haploid C. albicans biofilm model provides a new toolbox to uncover the mechanism governing the higher antifungal tolerance of biofilms. Here, we comprehensively examined the proteomics and antifungal susceptibility of standard diploid (SC5314 and BWP17) and stable haploid (GZY792 and GZY803) strains of C. albicans biofilms. Subsequent downstream analyses identified alkyl hydroperoxide reductase 1 (AHP1) as a critical determinant of C. albicans biofilm's tolerance of amphotericin B. At 32 μg/ml of amphotericin B, GZY803 haploid biofilms showed 0.1% of persister population as compared with 1% of the diploid biofilms. AHP1 expression was found to be lower in GZY803 biofilms, and AHP1 overexpression in GZY803 restored the percentage of persister population. Consistently, deleting AHP1 in the diploid strain BWP17 caused a similar increase in amphotericin B susceptibility. AHP1 expression was also positively correlated with the antioxidant potential. Furthermore, C. albicans ira2Δ/Δ biofilms were susceptible to amphotericin B and had a diminished antioxidant capacity. Interestingly, AHP1 overexpression in the ira2Δ/Δ strain restored the antioxidant potential and enhanced the persister population against amphotericin B, and shutting down the AHP1 expression in ira2Δ/Δ biofilms reversed the effect. In conclusion, we provide evidence that the AHP1 gene critically determines the amphotericin B tolerance of C. albicans biofilms possibly by maintaining the persisters' antioxidant capacity. This finding will open up new avenues for developing therapies targeting the persister population of C. albicans biofilms. The mass spectrometry proteomics data are available via ProteomeXchange with identifier PXD004274.
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