ArticleAnalytical chemistry2016
Single-Cell, Multiplexed Protein Detection of Rare Tumor Cells Based on a Beads-on-Barcode Antibody Microarray.
Article in Analytical chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Single-Cell Protein Assays in Context: From 2D to 3D and In Situ Analysis.Annual review of analytical chemistry (Palo Alto, Calif.) · 2026Review
- Single-cell omics: experimental workflow, data analyses and applications.Science China. Life sciences · 2025Review
- Photosensitive Hydrogel with Temperature-Controlled Reversible Nano-Apertures for Single-Cell Protein Analysis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Development of constrictional microchannels and the recurrent neural network in single-cell protein analysis.Frontiers in bioengineering and biotechnology · 2023Article
- The Intriguing Landscape of Single-Cell Protein Analysis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Review
- The future of microfluidics in immune checkpoint blockade.Cancer gene therapy · 2021Review
- Single-Cell Proteomics.Trends in biochemical sciences · 2021Review
- Deep Profiling of Cellular Heterogeneity by Emerging Single-Cell Proteomic Technologies.Proteomics · 2020Review
- Microfluidic single-cell analysis-Toward integration and total on-chip analysis.Biomicrofluidics · 2020Review
- Injection Molded Microfluidics for Establishing High-Density Single Cell Arrays in an Open Hydrogel Format.Analytical chemistry · 2020Article
- Single-Cell Analysis of Foot-and-Mouth Disease Virus.Frontiers in microbiology · 2020Review
- Ultrasimple Single-Cell Detection of Multiple Cytokines by a Nanowell Chip Integrated with Encoded Microarrays.ACS sensors · 2019Article
- Perspective of Molecular Diagnosis in Healthcare: From Barcode to Pattern Recognition.Diagnostics (Basel, Switzerland) · 2019Article
- Single-cell protein profiling in microchambers with barcoded beads.Microsystems & nanoengineering · 2019Article
- Microparticle Delivery of Protein Markers for Single-Cell Western Blotting from Microwells.Small (Weinheim an der Bergstrasse, Germany) · 2018Article
- Proteome Profiling of 1 to 5 Spiked Circulating Tumor Cells Isolated from Whole Blood Using Immunodensity Enrichment, Laser Capture Microdissection, Nanodroplet Sample Processing, and Ultrasensitive nanoLC-MS.Analytical chemistry · 2018Article
- Defining Cell Identity with Single-Cell Omics.Proteomics · 2018Review
- Highly Multiplexed Single-Cell Protein Profiling with Large-Scale Convertible DNA-Antibody Barcoded Arrays.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2018Article
- Single-cell technologies for profiling T cells to enable monitoring of immunotherapies.Current opinion in chemical engineering · 2018Article
- Single cell proteomics in biomedicine: High-dimensional data acquisition, visualization, and analysis.Proteomics · 2017Review
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Authors and funding
6 authors.
Funding
Abstract
Circulating tumor cells (CTCs) shed from tumor sites and represent the molecular characteristics of the tumor. Besides genetic and transcriptional characterization, it is important to profile a panel of proteins with single-cell precision for resolving CTCs' phenotype, organ-of-origin, and drug targets. We describe a new technology that enables profiling multiple protein markers of extraordinarily rare tumor cells at the single-cell level. This technology integrates a microchip consisting of 15000 60 pL-sized microwells and a novel beads-on-barcode antibody microarray (BOBarray). The BOBarray allows for multiplexed protein detection by assigning two independent identifiers (bead size and fluorescent color) of the beads to each protein. Four bead sizes (1.75, 3, 4.5, and 6 μm) and three colors (blue, green, and yellow) are utilized to encode up to 12 different proteins. The miniaturized BOBarray can fit an array of 60 pL-sized microwells that isolate single cells for cell lysis and the subsequent detection of protein markers. An enclosed 60 pL-sized microchamber defines a high concentration of proteins released from lysed single cells, leading to single-cell resolution of protein detection. The protein markers assayed in this study include organ-specific markers and drug targets that help to characterize the organ-of-origin and drug targets of isolated rare tumor cells from blood samples. This new approach enables handling a very small number of cells and achieves single-cell, multiplexed protein detection without loss of rare but clinically important tumor cells.
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