Evidence map›Paper›PMID 27644158›Full record

ArticleScientific reports2016

Candida albicans Dbf4-dependent Cdc7 kinase plays a novel role in the inhibition of hyphal development.

Wei-Chung Lai, Tschen-Wei Chang, Chang Hao Wu, Shu-Ya Yang, Tai-Lin Lee, Wan Chen Li, Ting Chien, Yu-Che Cheng, Jia-Ching Shieh

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.7field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Filament Negative RegulatorJournal of fungi (Basel, Switzerland) · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Wei-Chung LaiDepartment of Biomedical Sciences, Chung Shan Medical University, Taichung City, Taiwan, Republic of China.
Tschen-Wei ChangDepartment of Biomedical Sciences, Chung Shan Medical University, Taichung City, Taiwan, Republic of China.
Chang Hao WuDepartment of Biomedical Sciences, Chung Shan Medical University, Taichung City, Taiwan, Republic of China.
Shu-Ya YangDepartment of Biomedical Sciences, Chung Shan Medical University, Taichung City, Taiwan, Republic of China.
Tai-Lin LeeDepartment of Molecular Biotechnology, Da-Yah University, Changhua County, Taiwan, Republic of China.
Wan Chen LiDepartment of Biomedical Sciences, Chung Shan Medical University, Taichung City, Taiwan, Republic of China.
Ting ChienDepartment of Biomedical Sciences, Chung Shan Medical University, Taichung City, Taiwan, Republic of China.
Yu-Che ChengDepartment of Biomedical Sciences, Chung Shan Medical University, Taichung City, Taiwan, Republic of China.
Jia-Ching ShiehDepartment of Biomedical Sciences, Chung Shan Medical University, Taichung City, Taiwan, Republic of China.
Chung Shan Medical University · TWChung Shan Medical University Hospital · TWDayeh University · TW

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Candida albicans is an opportunistic human fungal pathogen. The ability to switch among multiple cellular forms is key to its pathogenesis. The Dbf4-dependent protein kinase gene CDC7 is conserved due to its role in initiating DNA replication. Because a C. albicans Cdc7 (Cacdc7) homozygous null was not viable, we generated a C. albicans strain with a deleted C. albicans CDC7 (CaCDC7) allele and an expression-repressible allele. Surprisingly, cells of the strain grew as hyphae under the repressed conditions. The in vitro kinase assays confirmed that CaCdc7 (K232) and CaCdc7 (T437) are critical for catalytic and phosphoacceptor of activation activity, respectively. C. albicans cells formed hyphae when expressing either the catalytically inactive CaCdc7 (K232R) or the phosphoacceptor-deficient CaCdc7 (T437A). While CaCdc7 interacted with CaDbf4, cells of the strain in which CaCDC7 was repressed were not rescued by constitutively expressing C. albicans DBF4 or vice versa. We conclude that CaDBF4-dependent CaCDC7 is an essential gene suppressing the hyphal development.

Indexed as

Candida albicansCell Cycle ProteinsDNA, FungalDNA ReplicationFungal ProteinsHyphaeCell Cycle ProteinsDNA, FungalFungal Proteins

Identifiers

PMID27644158
PMCPMC5028767
OpenAlexW2521709510

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.