ArticlePloS one2016
Linkage between Fitness of Yeast Cells and Adenylate Kinase Catalysis.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 17 citations in OpenAlex.
- Comprehensive proteomics analysis of bovine sperm head plasma membrane associated with fertility.Scientific reports · 2026Article
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- Changes in flexibility but not in compactness underlie the thermal adaptation of prokaryotic adenylate kinases.Evolution letters · 2025Article
- Magnesium induced structural reorganization in the active site of adenylate kinase.Science advances · 2024Article
- Elucidating Dynamics of Adenylate Kinase from Enzyme Opening to Ligand Release.Journal of chemical information and modeling · 2024Article
- Mechanistic Basis for a Connection between the Catalytic Step and Slow Opening Dynamics of Adenylate Kinase.Journal of chemical information and modeling · 2023Article
- Protein dynamics: The future is bright and complicated!Structural dynamics (Melville, N.Y.) · 2023Article
- Insights into the evolution of enzymatic specificity and catalysis: From Asgard archaea to human adenylate kinases.Science advances · 2022Article
- Proteomic Shifts Reflecting Oxidative Stress and Reduced Capacity for Protein Synthesis, and Alterations to Mitochondrial Membranes inMicroorganisms · 2022Article
- Article
- Overexpression of Phosphoribosyl Pyrophosphate Synthase Enhances Resistance ofFrontiers in plant science · 2021Article
- Cell cycle-dependent association of polo kinase Cdc5 with CENP-A contributes to faithful chromosome segregation in budding yeast.Molecular biology of the cell · 2019Article
- Molecular mechanism of ATP versus GTP selectivity of adenylate kinase.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- The Effect of Fermented Lingonberry Juice onInternational journal of dentistry · 2017Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Enzymes have evolved with highly specific values of their catalytic parameters kcat and KM. This poses fundamental biological questions about the selection pressures responsible for evolutionary tuning of these parameters. Here we are address these questions for the enzyme adenylate kinase (Adk) in eukaryotic yeast cells. A plasmid shuffling system was developed to allow quantification of relative fitness (calculated from growth rates) of yeast in response to perturbations of Adk activity introduced through mutations. Biophysical characterization verified that all variants studied were properly folded and that the mutations did not cause any substantial differences to thermal stability. We found that cytosolic Adk is essential for yeast viability in our strain background and that viability could not be restored with a catalytically dead, although properly folded Adk variant. There exist a massive overcapacity of Adk catalytic activity and only 12% of the wild type kcat is required for optimal growth at the stress condition 20°C. In summary, the approach developed here has provided new insights into the evolutionary tuning of kcat for Adk in a eukaryotic organism. The developed methodology may also become useful for uncovering new aspects of active site dynamics and also in enzyme design since a large library of enzyme variants can be screened rapidly by identifying viable colonies.
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