ArticleOxidative medicine and cellular longevity2016
Differential Regulation of the Extracellular Cysteine/Cystine Redox State (EhCySS) by Lung Fibroblasts from Young and Old Mice.
Article in Oxidative medicine and cellular longevity, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 16 citations in OpenAlex.
- α4 Nicotinic Acetylcholine Receptors in Lipopolysaccharide-Related Lung Inflammation.International journal of molecular sciences · 2024Article
- The profibrotic and senescence phenotype of old lung fibroblasts is reversed or ameliorated by genetic and pharmacological manipulation of Slc7a11 expression.American journal of physiology. Lung cellular and molecular physiology · 2022Article
- Ginkgolic acid improves bleomycin-induced pulmonary fibrosis by inhibiting SMAD4 SUMOylation.Oxidative medicine and cellular longevity · 2022Article
- Na/H Exchange Regulatory Factor 1 Deficient Mice Show Evidence of Oxidative Stress and Altered Cisplatin Pharmacokinetics.Antioxidants (Basel, Switzerland) · 2021Article
- Redox States of Protein Cysteines in Pathways of Protein Turnover and Cytoskeleton Dynamics Are Changed with Aging and Reversed by Slc7a11 Restoration in Mouse Lung Fibroblasts.Oxidative medicine and cellular longevity · 2020Article
- Plasma cysteine/cystine and glutathione/glutathione disulfide redox potentials in HIV and COPD patients.Free radical biology & medicine · 2019Article
- Oxidative Toxicology of Bleomycin: Role of the Extracellular Redox Environment.Current opinion in toxicology · 2019Article
- Maternal undernutrition during pregnancy alters the epigenetic landscape and the expression of endothelial function genes in male progeny.Nutrition research (New York, N.Y.) · 2019Article
- Age-dependent oxidation of extracellular cysteine/cystine redox state (EFree radical biology & medicine · 2018Article
- Follicular Fluid redox involvement for ovarian follicle growth.Journal of ovarian research · 2017Review
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6 authors at 1 institution in 1 country.
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Abstract
Aging is associated with progressive oxidation of plasma cysteine (Cys)/cystine (CySS) redox state, expressed as EhCySS. Cultured cells condition their media to reproduce physiological EhCySS, but it is unknown whether aged cells produce a more oxidized extracellular environment reflective of that seen in vivo. In the current study, we isolated primary lung fibroblasts from young and old female mice and measured the media EhCySS before and after challenge with Cys or CySS. We also measured expression of genes related to redox regulation and fibroblast function. These studies revealed that old fibroblasts produced a more oxidizing extracellular EhCySS than young fibroblasts and that old fibroblasts had a decreased capacity to recover from an oxidative challenge due to a slower rate of reduction of CySS to Cys. These defects were associated with 10-fold lower expression of the Slc7a11 subunit of the xCT cystine-glutamate transporter. Extracellular superoxide dismutase (Sod3) was the only antioxidant or thiol-disulfide regulating enzyme among 36 examined that was downregulated in old fibroblasts by more than 2-fold, but there were numerous changes in extracellular matrix components. Thus, aging fibroblasts not only contribute to remodeling of the extracellular matrix but also have a profound effect on the extracellular redox environment.
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